Fig. 5

CircSLCO1B3 regulated cholangiocarcinoma growth depend on HOXC8. a, b, c, d Relative mRNA and protein levels of circSLCO1B3, HOXC8 and SMAD3 in ICC cells transfected with overexpression circSLCO1B3 vectors and HOXC8 siRNA were assessed by qPCR and western blotting. e, f, g, h, i, j, k, l, m CCK-8 assays, colony formation assays and EdU assays were performed to determine the ability of proliferation in RBE and HuCCT1 cells transfected with circSLCO1B3 vectors and HOXC8 siRNA (magnification, × 200, scale bar, 100 μm). n, o, p, q, r, s, t, u Trans-well assays and wound healing assays were conducted to detect the migratory and invasive capabilities in RBE and HuCCT1 cells transfected with circSLCO1B3 vectors and HOXC8 siRNA (magnification, × 200, scale bar, 100 μm). Data were showed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001