Fig. 6

METTL3-mediated m6A modification enhanced circSLCO1B3 stability via YTHDC1. a, b, c MeRIP analysis of the abundance of m6A-modified circSLCO1B3 in RBE and HuCCT1 cells. d qPCR was performed to determine the key regulator of m6A modification in circSLCO1B3 regulation. e, f, qPCR was performed to determine the expression of methyltransferase METTL3 in CCA cells and tissues. g, h RIP assays were executed to explore the enrichment of circSLCO1B3 by the anti-METTL3 antibody. i, j, k, l Colony formation assays were performed to determine the ability of proliferation in RBE and HuCCT1 cells transfected with METTL3 siRNA and circSLCO1B3 vectors. m, n, o, p, q, r Transwell assays were conducted to detect the migratory and invasive capabilities in RBE and HuCCT1 cells transfected with METTL3 siRNA and circSLCO1B3 vectors (magnification, × 200, scale bar, 100 μm). s qPCR was conducted to detect the circSLCO1B3 expression levels in HuCCT1 cells transfected with YTHDC1 siRNA. t, u RIP assays were executed to explore the enrichment of circSLCO1B3 by the anti-YTHDC1 antibody. v Relative RNA levels of circSLCO1B3, SLCO1B3, and pre-SLCO1B3 upon YTHDC1 knockdown in HuCCT1 cells. w Actinomycin D RNA stability assays were conducted in HuCCT1 after transfecting with YTHDC1 siRNA. x, y The expression levels of circSLCO1B3 after simultaneously transfecting with YTHDC1 and METTL3 siRNA. Data were showed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001