Fig. 7

CircSLCO1B3 promoted cholangiocarcinoma progression in vivo. a The process of constructing subcutaneous xenograft tumor in nude mice. b, c Mice weight and tumor volumes were monitored every 2 days. Data are mean ± SD and p < 0.05 by one-way ANOVA followed by Tukey’s multiple comparisons test. d Stably transfected HuCCT1 cells from different groups were inoculated into BALB/c nude mice to establish subcutaneous xenograft tumors (n = 8 mice/group). e Images of xenograft tumors of each group (n = 8). f Tumors weight in three groups. g, h, i, g, k IHC staining show the relative levels of HOXC8, SMAD3, N-cadherin and ki67 in tumors from different groups. Scale bar, 50 μm. Mean ± SEM. Student's t-test. l The process of constructing metastasis xenograft tumour in nude mice. m The body weight of the three groups of nude mice. n Bioluminescent images of lungs for each experimental group. o Pathology of the lung tissue was detected by HE staining. *P < 0.05, 0.001 < ** P < 0.01, *** P < 0.001