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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: CDKL1 potentiates the antitumor efficacy of radioimmunotherapy by binding to transcription factor YBX1 and blocking PD-L1 expression in lung cancer

Fig. 3

CDKL1 interacts with YBX1 in vitro and in vivo. (A) The proteins that interact with CDKL1 were identified by screening using tandem affinity purification (TAP) and mass spectrometry (MS). (B) The interaction between exogenous CDKL1 and exogenous YBX1 was assessed in HEK293T cells. (C) The interaction between exogenous CDKL1 and endogenous YBX1 was assessed in A549 and H1299 cells. (D) In H1299 cells, the endogenous interaction between CDKL1 and YBX1 was examined using anti-IgG and anti-YBX1 antibodies. (E) GST-only and GST-CDKL1 proteins were isolated from bacteria. Proteins extracted from HEK293T cells transfected with SFB-YBX1 were incubated with GST-only or GST-CDKL1 overnight, and their presence was detected by immunoblotting

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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