Fig. 6
CDKL1 overexpression inhibits immune evasion in vivo and in vitro, and triple therapy yields the most effective antitumor outcomes in lung cancer. (A) The percentage changes in IFN-γ and GZMB levels in each group were analyzed using flow cytometry in vitro (** P < 0.01, *** P < 0.001). (B) Images of xenograft tumors in C57BL/6J mice. Tumors were measured for volume determination every three days (n = 8, *** P < 0.001). (C) Tumor wet weight was determined (n = 8, ** P < 0.01). (D) The percentage of CD8+ T cells among CD45+ cells, as well as the levels of IFN-γ and GZMB, were assessed in the subcutaneously transplanted tumors on day 14 after inoculation (* P < 0.05, ** P < 0.01, *** P < 0.001). (E) A schematic diagram illustrating the treatment protocol. (F) The mice were randomly divided into nine groups and subjected to treatment. Tumors were measured for volume determination every three days (n = 7). (G) The percentage of CD8+ T cells among CD45+ cells within the subcutaneously transplanted tumors was assessed on day 25 after treatment with various therapies (n = 7). (H) The amount of IFN-γ secreted by CD8+ T cells within the subcutaneously transplanted tumors was determined on day 25 post inoculation (n = 7)