Fig. 3
From: Anaplastic thyroid cancer spheroids as preclinical models to test therapeutics
Illustration of ATC spheroid generation using Matrigel droplets and representative checkpoint images. (A) 3D Matrigel cell suspension droplets were used to generate ATC tumor spheroids. ATC cells were resuspended in ice-cold Matrigel liquid, then placed on a 37 ℃ preheated Petri dish as individual droplets. The tumor cell droplets were cultured in the medium and the dish was placed on an orbital shaker (120 rpm) in a 37 ℃ incubator (5.0% CO2); the medium was changed every one to three days. Representative images were taken at several time points. Scale bars, 200 μm. (B) Morphologies of developing ATC tumor spheroids generated from different human cancer cells. Left panel: representative bright-field images of ATC tumor spheroids derived from BRAFV600E-mutant cell lines (8505 C, SW1736), BRAFWT cell lines (C6543, THJ-16T), and patient-derived cells (ATC01) throughout the time course. Right panel: high-magnification view of colony formation and cell invasion within the ATC tumor spheroid at day 14. Discohesive and cohesive growth patterns were presented in the ATC spheroids. Scale bars, 100 μm. Medium A = DMEM/F12 medium, 15% FBS, 1% GlutaMAX, 1% antibiotic-Pen/strep, and 5 µM ROCK inhibitor (Y-27,632); Medium B = DMEM/F12 medium, 10% FBS, and 1% GlutaMAX