Fig. 6
From: Anaplastic thyroid cancer spheroids as preclinical models to test therapeutics
Comparison of gene expression between ATC cells from monolayer culture, spheroids culture, and parental tumor sample. (A) Heatmap showing gene expressions in ATC tumors and the matched monolayers and 3D spheroids. Genes with expressions that (a) have no significant difference between ATC tumors and matched 3D spheroids and (b) have a significant difference when comparing ATC tumors/matched 3D spheroids versus matched monolayers were selected. Red color represents higher expression while blue represents lower expression. (B) Heatmap showing upregulated gene expressions from comparison of 3D spheroids versus monolayers across 8505Â C ATC cell line, C643 ATC cell line, and patient-derived ATC01 cells. (C) The RNA expression level of CDH1 and CDH2 in ATC01 cells. (D) The RNA expression level of CDH1 and CDH2 in 8505Â C and C643 cells. (E) Pathway enrichment analysis for upregulated genes from (A). (F) Pathway enrichment analysis for upregulated genes from (B)