Fig. 1

Cholesterol metabolism was pivotal for TNBC progression. (a) Schematic representation of the in vivo screen using the mouse genome-scale CRISPR-Pool SAM library. 4T1 cells were infected with lentiviral CRISPR-Pool SAM library, and subcutaneously (s.c.) inoculated into mice. CD8⁺ T cells were depleted by injecting CD8 antibodies one day prior to tumor inoculation to allow tumor formation. Tumors were collected on days 5 and day 10 for sgRNA sequencing. (b) Bubble graph depicting the Gene Ontology (GO) enrichment analysis of gene sets in tumor tissues between day 5 and day 10. (c) 4T1 cells were s.c. inoculated into BALB/c mice and fed with a western diet, high-cholesterol diet, or normal diet. Mice were sacrificed on day 14 (n = 5) and the metastatic nodes were counted. (d) Representative images of H&E staining of lung and liver tissues from mice. (e) Serum was collected on day 10 and cholesterol (CHOL), low-density lipoprotein (LDL), high-density lipoprotein (HDL) and Triglyceride (TG) levels were measured. (f) Survival of mice receiving different diets was monitored. (g) Relative mRNA levels of krt14 in tumor tissues were measured using qPCR. *p < 0.05; **p < 0.01; ***p < 0.001