Fig. 5

I3A induced B7-H3 expression enhances B7-H3-CAR T cell effector function. a CD19-CAR (Control) or B7-H3-CAR T cell IFNγ (left panel) and IL2 (right panel) secretion 24 h post coculture with LM7-KO or LM7 cells treated with DMSO or 0.1 µM I3A (N = 3). b Diagram of cytotoxicity assay. c CD19-CAR or B7-H3-CAR T cell killing of LM7-KO or LM7 cells treated with DMSO or 0.1 µM I3A (N = 3). Data represents mean ± SEM (a and c). *p < 0.05, ****p < 0.0001, ns, non-significant by two-way ANOVA (a and c)