Fig. 4

GPR65 was the principal receptor mediating lactate stimulation in TAMs in glioma. A Differential mRNA expression levels of GPR65 among GBM, LGG, and Normal groups in TCGA dataset. B Kaplan–Meier survival curves for correlation between GPR65 mRNA expression and survival of glioma patients in the TCGA dataset. C Western blot analysis and quantification of GPR65 protein expression in clinical samples. D-E Cellular distribution of GPR65 in glioma using single-cell RNA-seq datasets from UCSC Cell Browser (https://gbm.cells.ucsc.edu) and Single Cell Portal (https://singlecell.broadinstitute.org, GSE131928). F Representative pictures and quantification for immunofluorescence staining of clinical tissues for GPR65, and / CD68 or CD206. Data are presented as the Mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001