Fig. 2
Characterization of the immune infiltrate in bladder CIS at baseline in patients achieving or not a CR after the intensive phase. a Intra-tumoral density (cells/mm2) of CD8+ T cells. b (Left) Representative image of a bladder CIS sample stained with the first mIF panel. In the crop, the proximity between CD8+ cells (magenta staining) and a CD4 + FoxP3+ cell (white and green staining) is highlighted. Original magnification × 20. (Right) Percentage of CD8+ T cells within a radius of 20 μm from CD4 + FoxP3+ Treg cells within the tumor regions. c (Left) Representative image of a bladder CIS sample stained with the first mIF panel. The color code is the same as in (b). In the crop, the proximity between a CD68 + CD163+ macrophage (grey and orange staining) and CD8+ cells (magenta staining) is highlighted. Original magnification × 20. (Right) Percentage of CD163+ M2-polarized macrophages within a radius of 20 μm from CD8+ T lymphocytes. d Percentage of CD68 + CD163- macrophages within a radius of 20 μm from CD8+ T lymphocytes within the tumor regions. e Mean distance (μm) between each CD68 + CD163- macrophage and the nearest CD8+ T lymphocyte. Significantly different data are represented by *p < 0.05. Floating box extends from 25th to 75th percentiles, line through the box indicates median, and bars extend from the smallest to largest values. f Schematic representation of the analysis of the number and type of cell interactions carried out progressively moving away from the tumor margin. Bubble graphs show the percentage of cell-to-cell interactions (dimension of the bubbles), progressively moving away from the tumor margin in CRIP and NRIP patients