Fig. 1

Mutation spectrum of RBM10 in CCA. A Functional cluster analysis was conducted in our previously identified potential driver genes in CCA. The gene symbols in red represented the CCA-related genes that were identified in our previous study. Gene symbols in black were previously reported in CCA. NMU, our own CCA cohort (67 cases); ICGC, International Cancer Genome Consortium (179 cases); NC Zou et al.’s study [38] (102 cases). B RBM10 alteration frequency in pan-cancer in Chinese population from Cibioportal. C Top 30 Concurrent gene alterations in CCA patients with RBM10 mutations in the Chinese population. D Significant alteration rate difference between RBM10 alter and unalter groups in CCA (P < 0.05). Blue column RBM10 unalter group; red column, RBM10 alter group. E RBM10 point mutation distribution in 58 of 859 CCA samples in the Chinese population. Lollipop plot showed mutations throughout the RBM10 gene area, using MutationMapper. Green rectangle, two RNA recognition motifs (RRM); red rectangle, two zinc fingers (left, C4-type zinc finger domain; Right, C2H2-type zinc finger domain); purple rectangle, Octamer repeat domain(OCRE). F RBM10 point mutation distribution in 5 of 67 CCA samples from our dataset. G Pathogenicity prediction of mutations in our cohort using PROVEAN and SIFT