Fig. 3
RBM10 was a tumor suppressor and RBM10C761Y was a loss-of-function mutation in CCA. A Sanger sequencing plot of interested region including RBM10 C761Y in QBC939 and RBE. B, C Establishment of stable WT and MUT cells was evaluated at mRNA and protein levels. NC, negative control; WT, wild-type; MUT, mutant. D, E, F Proliferation ability of different groups was examined by EdU, CCK-8, and colony formation assay. G, H Migration ability of different groups was detected by wound-healing and transwell assay. I Photograph of excised tumors from mice in different groups (n = 5). J Representative images showed H&E and IHC staining for RBM10 and Ki67 in tumors removed from nude mice