Fig. 7

ASPM203 interacted with DVL2 to facilitate Wnt/β-catenin signaling. A The diagram illustrates the role of ASPM in regulating the Wnt signaling pathway, as reported in previous studies. B Co-immunoprecipitation was performed to verify the physical interaction between ASPM203 and dishevelled-2 (DVL2), a key mediator of Wnt/β-catenin signaling. C, D Using RT-PCR and western blot to determine the different levels of Wnt/β-catenin signaling associated genes in the NC, WT, and MUT groups. E, F Using RT-PCR and western blot to determine the different levels of Wnt/β-catenin signaling associated genes with knocking down of ASPM203 in the NC group. G, H Expression and ubiquitylation of DVL2 were detected in four groups, including the NC, WT, MUT, and ASPM203-knockdown groups