Fig. 8

ASPM203 promoted CCA progression in a Wnt/β-catenin signaling dependent manner. A, B, C The alterations of CCA cell lines proliferation were evaluated by EdU, CCK-8, and colony formation assay in MUT, ASPM203-knockdown, and ASPM203-knockdown with DVL2-overexpression groups. D, E The alterations of CCA cell line migration were assessed by wound healing and transwell assay in MUT, ASPM203-knockdown, and ASPM203-knockdown with DVL2-overexpression groups. F The efficiency of ASPM203 knockdown and DVL2 overexpression were evaluated by western blotting. G Schematic representation of a model that RBM10 C761Y mutation induced oncogenic ASPM isoforms and regulated Wnt/β-catenin signaling in CCA