Fig. 10

Nascent RNA sequencing revealed a p60AMOTL2-dependent transcriptional response to BET inhibition. A The Venn diagram illustrates the dependence of BET inhibitor-induced transcriptional change on p60AMOTL2, highlighting 655 differentially expressed genes (DEGs) in p60AmotL2-expressing cells in contrast to only 78 DEGs in control cells after iBET151 treatment, based on exon feature unique counts. An adjusted p-value cutoff < 0.1 was used. B and C Functional enrichment analyses of the DEGs in p60AmotL2-expressing and non-expressing cells after iBET151 inhibition for Gene Ontology (GO) terms and Gene Set Enrichment Analysis (GSEA), with significant hallmark gene sets found only in p60AMOTL2 expressing cells (< 0.05 p-adj, BH)), respectively. These analyses revealed a significant shift towards pro-apoptotic pathways associated with p53 signalling. An adjusted p-value cutoff < 0,05 was used