Fig. 2

RNA-seq analysis of p60AmotL2-induced gene expression in MDCK cells. RNA expression was analysed in MDCK cells after Dox induction of p60AmotL2 expression. To exclude the influence of Dox on gene expression, Dox-treated control cells were analysed in parallel (Supplemental Data 1). A Volcano plot to visualize the results of differential expression between p60AmotL2 \(+\) and p60AmotL2 \(-\) cells. Label description: NS – non significant; FC – fold change; P – p-value. B Identification of KEGG pathways affected by p60AmotL2 expression. The size of each circle represents the number of transcripts involved in the corresponding pathway, and the color scale is related to the q-value. C and D RNA-seq heatmap analysis provides a visual overview of KEGG pathways identified in B. E Immunofluorescence staining using MAB414 monoclonal antibody, which recognizes a family of nuclear pore complex (NPC) proteins. Note the decreased fluorescent intensity of nuclear pore-associated MAB414 antibodies in p60AmotL2-expressing cells. F Scatter plot showing quantification of the immunofluorescence signal of MAB414 in the indicated conditions (n = 142, corresponding to individual nuclei; Student’s T test, *** p < 0.001). Data represent the mean ± SD from three independent experiments. Scale bar = 5 μm