Fig. 4

miR-4732-3p is sorted into fucosylated exosomes via hnRNPK. A Sequence motifs of hnRNPK binding site predicted by RBPsuite. B RIP assay using hnRNPK antibody and qRT-PCR were performed, anti-IgG as the negative control. C miRNA pull-down and western blot analysis were conducted to confirm interaction between miR-4732-3p and hnRNPK protein. D Western blot analysis was applied to define knockdown efficiency of hnRNPK in NSCLC cells transfected with si-hnRNPK. E–F qRT-PCR analysis of miR-4732-3p levels in NSCLC cells (E), and their corresponding fucosylated exosomes (F) following hnRNPK knockdown. G qRT-PCR analysis was performed to determine the effects of miR-4732-3p mimics and si-hnRNPK on the levels of fucosylated exosomal miR-4732-3p. Data are shown as the mean ± SD from at least three independent experiments. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001; ^****p < 0.0001; ns, not significant