Fig. 1

SF3B3 expression is upregulated in human CRC. A SF3B3 transcript levels in normal and CRC tissues. The data were obtained from TCGA-COAD and TCGA-READ datasets in Xena (http://xena.ucsc.edu/). B Relative SF3B3 mRNA levels in CRC and pair matched adjacent normal tissues. The data were obtained from TCGA-COAD and TCGA-READ datasets in Xena, as well as GEO datasets. C Relative SF3B3 mRNA levels in paired CRC and adjacent normal tissues. The mRNA expression was determined by qRT-PCR. D Western blot analysis of SF3B3 protein in paired CRC and adjacent normal tissues. E Representative IHC staining images of SF3B3 protein and statistical analysis of SF3B3 IHC scores in CRC and adjacent normal tissues. The staining extent was scored on a scale of 0 − 4. The staining intensity was scored at 0–3 (3 is the highest positivity). The final IHC score was generated by multiplying the score of staining extent with the score of staining intensity. The maximum score is 12. F Kaplan–Meier analysis of overall survival from CRC patients with high and low SF3B3 protein levels (n = 29). G Representative IHC staining images of SF3B3 protein and statistical analysis of SF3B3 IHC scores in primary CRC and their corresponding liver metastasis tissues. The final IHC score was generated by multiplying the score of staining extent with the score of staining intensity. H ChIP-PCR analysis of the enrichment of H3K27ac in SF3B3 promoter region relative to IgG in LoVo cells. The immunoprecipitated DNA fragments by anti-H3K27ac and IgG were purified and analyzed by PCR with primers specific for SF3B3 promoter. I Genome browser view of H3K27ac ChIP-seq data in SF3B3 promoter region from CRC patient tissues. Data are shown as mean ± SD