Fig. 4

SF3B3 regulates lipogenesis in CRC cells via SREBF1-FASN signaling. A Heatmap displaying the top 10 up- and down-regulated genes from RNA-seq analysis of LoVo cells transiently transfected with siRNAs (siSF3B3#1 + siSF3B3#2) for 48 h. B KEGG pathway analysis of SF3B3-regulated genes from RNA-seq. C Schematic representation of SREBF1-dependent lipogenesis. D mRNA expression of SREBF1 and its target genes, as well as representative western blots of the proteins in SF3B3-knockdown CRC cells. mRNA levels were detected at 48 h, and protein levels were detected at 72 h after transfection. E Representative western blots of SREBF1, ACLY, ACACA, FASN and SCD in LoVo xenografts (shNC vs shSF3B3#1). F Representative western blots of C-CASP3 and C-PARP in SF3B3-knockdown CRC cells. Cells were transfected with siRNAs for 24 h, then treated with or without 10 μM PA (palmitate) for 48 h. G Lipidomic profiles displaying the fatty acids in LoVo cells transiently transfected with siRNAs (siSF3B3#1 + siSF3B3#2) for 72 h. Schematic representation of long-chain fatty acids that were significantly decreased (in green) in SF3B3-knockdown LoVo cells. H Representative IHC images of FASN in CRC tissues and adjacent normal tissues. The final IHC score was generated by multiplying the score of staining extent with the score of staining intensity. Statistical analysis of FASN IHC scores and correlation analysis with SF3B3 in CRC tissues. Data are shown as mean ± SD