In GFP with high risk HPV-18E6 fusion protein expressed 293T and MCF-7 cells, the endogenous wild-type p53 could be transiently phosphorylated at multiple sites
- Lina Sun†1, 2,
- Ge Zhang†3, 4,
- Zongfang Li3,
- Tusheng Song5,
- Chen Huang5Email author and
- Lusheng Si1, 6Email author
© Sun et al; licensee BioMed Central Ltd. 2008
Received: 11 July 2008
Accepted: 08 September 2008
Published: 08 September 2008
Infected cells recognize viral replication as a DNA damage stress and elicit the host surveillance mechanism to anti-virus infection. Modulation of the activity of tumor suppressor p53 is a key event in the replication of many viruses. They could manipulate p53 function through phosphorylation modification for their own purpose. But there is rarely research about p53 phosphorylation status in the context of HPV-E6. Therefore, we investigated whether p53 could be phosphorylated by HPV-E6.
We used a mammalian green fluorescence protein (GFP) expression system to express HPV-18E6 with GFP fusion proteins (GFP-18E6) in wild-type (wt) p53 cell lines, such as 293T and MCF-7 cells to trace the traffic and subcellular location of E6 protein. By immunofluorescence technique and immunoblotting, we determined the positive phosphorylated sites of p53 and observed the distribution of phosphorylated p53 in the context of GFP-18E6.
GFP-18E6 was predominantly located in nuclei of wt p53 cell lines, and it could induce transient phosphorylation of p53 at multiple sites, such as Ser15, Ser20, and Ser392. All the three sites of phosphorylated p53s were localized in nuclei together with GFP-18E6.
In GFP with high risk HPV-18E6 fusion protein expressed 293T and MCF-7 cells, the endogenous wt p53 could be transiently phosphorylated at multiple sites.
Human papillomaviruses (HPVs) are small double-stranded DNA viruses with a genome of approximately 8 kb [1, 2]. Over 90% of human cervical carcinoma is associated with high risk mucosal HPVs, mainly the serotypes 18 and 16 . The mechanisms underlying the actions of high risk HPVs leading to cancer have been studied extensively, and it was shown that the E6 and E7 proteins were the oncoproteins interacting with tumor suppressors p53 and pRb, respectively, and leading to infected-cell transformation and dysregulated proliferation [4, 5]. Previous studies also showed that the principle activity of E6 was to target and degrade p53, therefore, p53's growth regulatory functions is abolished . However, many authors reported the expression of E6 was not necessarily equated to a p53 null background [7–10]. Therefore, we hypothesized there might be other ways for E6 interaction with p53.
p53 is a very important tumor suppressor, it can be activated in response to DNA damage stresses [11–13]. Phosphorylation of p53 has been studied intensively and has been proposed to play a critical role in the stabilization and activation of p53 . Infected cells recognize viral replication as a DNA damage stress and elicit the host surveillance mechanism to anti-virus infection . The modulation of p53 function by phosphorylation seems to be a major antiviral defense mechanism employed by cells [16, 17]. On the other hand, some viruses have evolved strategies such as reducing the phosphorylation of p53 for counteraction p53 activation. For example, Kaposi's sarcoma associated herpesvirus (KSHV) is associated with the pathogenesis of Kaposi's sarcoma, KSHV viral interferon regulatory factor1 (vIRF1) greatly reduced the level of serine 15 phosphorylation of p53, resulting in an decrease of p53 stability which could circumvent host growth surveillance and facilitate viral replication in infected cells . But there is rarely research about p53 phosphorylation status in the context of HPV-E6.
Construction of expression vector
Full length HPV-18E6 sequence was amplified by PCR from HPV type 18 complete genome, and then cloned in frame within the C terminus of GFP at the Bgl II and EcoR I sites of the polylinker regions of the mammalian expression vector pGFP (Clontech, Palo Alto, CA), producing plasmid pGFP-18E6.
Cell culture and transfection
The human embryonic 293T kidney cells and human breast adenocarcinoma MCF-7 cells were maintained in RPMI1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere of 5% CO2. Cells were seeded on glass coverslips in 12-well cell culture plates. The cells were transiently transfected with plasmid pGFP -18E6, pGFP overnight using Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, Calif).
Cell imaging by fluorescent microscope
The 293T and MCF-7 cells were grown on glass coverslips, transfected, and fixed with 4% paraformaldehyde for 10 min at room temperature, rehydrated three times with cold PBS, then stained with DAPI (4',6-Diamidin-2'-phenylindoldihydrochlorid) at 37°C in the dark for 10 min, rinsed again with PBS and mounted on slides. Cell images were collected with a Nikon fluorescent microscope at a magnification of ×400. Fluorescent images were analyzed using Nikon Software.
For each sample, 106 cells were collected by centrifugation (1000 × rpm for 5 min), washed once with ice cold PBS, and lysed in 100 μl RIPA buffer containing 50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 1 mM EDTA, 2.5 mM glycerophosphate, 1 mM PMSF, 10 mM NaF, and phosphatase inhibitor cocktail (Roche Diagnostics, Mannheim Germany). Protein concentration was determined using the BCA reagents (Pierce, Rockford, IL). Samples (30 μg) were analyzed on 12% SDS polyacrylamide gels, transferred to PVDF membranes (Invitrogen), and blocked for 1 h at room temperature with 5% non-fat milk in TBS buffer (20 mM Tris-HCl [pH 7.5], 0.5 M NaCl). The membranes were then incubated with the primary antibody overnight at 4°C. After three washes with TBS, the membranes were incubated with the secondary antibody for 30 min at room temperature. After three additional washes, the proteins were visualized by enhanced chemiluminescence (ECL) (Amersham Pharmacia, Piscataway, New Jersey, USA).
The following primary antibodies were used: anti-phospho-p53 Ser6, anti-phospho-p53 Ser9, anti-phospho-p53 Ser15, anti-phospho-p53 Ser20, anti-phospho-p53 Ser37, anti-phospho-p53 Ser46, anti-phospho-p53 Ser 392, anti-chk2 (Cell Signaling; dilution, 1:1000) and anti-ATM (sigma; dilution,1:1000).
The cells were seeded on glass coverslips at a density of 1 × 105 cells/well. Then, they were transfected with plasmid pGFP -18E6 and pGFP overnight following standard procedures. After transfection, the cells were washed with PBS and fixed with 4% paraformaldehyde for 10 min at room temperature. They were then rehydrated three times with cold PBS, permeabilized with 1% Triton X-100 for 5 min on ice, and rinsed with PBS and blocked. The cells were incubated with primary antibodies overnight at 4°C. Subsequently, signal detection was performed using Cy3 -conjugated goat anti-rabbit IgG (Sigma; dilution, 1:200) in blocking solution for 30 min at room temperature in the dark. Then, the cells were washed three times with PBS and examined by confocal microscopy. Fluorescent images were analyzed using a Leica Confocal Software (Leica Microsystems).
All data were recorded as means ± standard deviation, and analyzed by the SPSS 11.0 software. Analysis of data was performed using one-way ANOVA for multiple comparisons. P values < 0.05 were considered statistically significant.
Results and discussion
GFP-18E6 was mainly located in nuclei
GFP-18E6 promoted multiple sites phosphorylation of p53 along with up-regulation of ATM and Chk2
Co-localization of GFP-18E6 and phosphorylated p53 proteins
Level of phosphorylated p53 along with time course
Since the expression of GFP-18E6 was associated with time course, we next determined the three sites of phosphorylated p53 level in 293T and MCF-7 cells from 12 h to 72 h post-transfection dynamically. For GFP-18E6 expressing cells, the Ser15, Ser20 of p53 were firstly detected at 12 h post-transfection and increased gradually, significant accumulation was observed at 24 h (P < 0.001). The expression level of Ser15 was higher than Ser20 at the same time point. It should be noted that phosphorylation of Ser392 was not present at 12 h in GFP-18E6 transfected MCF-7 cells, whereas it was highly expressed in GFP-18E6 transfected 293T at the same time. In both cells, the Ser392 reached highest level at 24 h post-transfection (P < 0.001). From 48 h to 72 h post-transfection, the three sites of phosphorylated p53s were not detected. As GFP control expressing cells, there was not phosphorylated p53 at Ser15, Ser20, and Ser392 during the whole period (Figure 4B). Thus, in 12 h to 24 h expression of GFP-18E6 there was a short term activation of p53 by phosphorylating modification. Because activation of p53 can also be modulated at the transcription level [22, 23], we next asked whether the mRNA level of p53 was increased by the expression of GFP-18E6. By reverse transcriptase (RT)-PCR, we clearly observed the mRNA of p53 was not changed in GFP-18E6 expressing cells (data not shown). This agreed with the mRNA level of p53 was stable in the context of HPV-E6 [24, 25].
Phosphorylation of p53 at Ser15 and Ser20 were the earliest response to E6 expression. It is general believed Ser15 phosphorylation of p53 occurs rapidly in response to DNA damage and appears to represent a 'priming event' for the subsequent series of modifications . Because phosphorylation of Ser15 induced by ATM/ATR (ATM-and-Rad3-related) results in dissociation of p53 from its negative regulator mdm-2, it has been suggested that the primary effect of phosphorylation of p53 at Ser15 is to increase p53 level . The Ser20 is also critical for stabilizing of p53. Recent studies have demonstrated that Ser20 on p53 is phosphorylated by Chk1 (checkpoint kinase 1) or Chk2, enhancing its tetramerization, stability, and activity in response to DNA damage . In fact, phosphorylated sites at the Ser15 and Ser20 residues lie right under the binding pocket of mdm-2, which could disrupt the binding with mdm-2, resulting stabilization of p53 . In the present study, the level of phosphorylation of p53 at Ser15 was clearly higher than Ser20. It's probably because Ser15 phosphorylation of p53 was a more important target than Ser20 in the context of HPV-18E6. This was consistent with some data reported that removing Ser15 can abrogate phosphorylation at Ser20 . For phosphorylation of p53 at Ser392, it was even not same for both cells. In 293T cells, phosphorylation of p53 at Ser392 appeared earlier and higher than MCF-7 cells. Thus, the different responses of Ser392 maybe due to varied sensitivity of cells. Authors reported phosphorylation of p53 at Ser392 was an early response to a wide range of stress-inducing conditions. Ser392 is phosphorylated by the protein kinase CK2 after both UV and ionizing radiation treatment . It has been shown to enable the transcriptional activation of the p53 protein in vitro and also seems to be important for p53-mediated transactivation in vivo [32, 33]. Therefore, the phorsphorylation of p53 at Ser15, Ser20, and Ser392 could stabilize and activate p53, which ultimately induces the irreversible cell cycle arrest and apoptosis in response of DNA damage stress .
It has been proved the tumor suppressor p53 could induce cell cycle arrest or apoptosis in response to stresses, such as UV radiation, DNA damage, hypoxia or virus infection [11, 12]. Previous also studies showed E6 could target and degrade p53, which in turn inhibited apoptosis . In present study, we clearly observed the phosphorylating modification of p53 in the early stage of HPV-18E6 expressing. The phosphorylation of p53 could induce apoptosis or cell cycle arrest in response to DNA damage stresses [29–31]. Regulation of p53 phosphorylation has also been shown to be induced by many viruses, such as, Africa swine fever virus (ASFV), the p53 in host cell is stabilized by phosphorylation at Ser392 and is located in the nuclei. During infection, the phosphorylated p53 is functionally active to induce apoptosis along with the expression of p21 and mdm2 . The Epstein-Barr virus (EBV) can activate p53 through phosphorylated modification at Ser15, Ser20, and Ser392 modulated by its oncogenic protein LMP1. Additionally, the phosphorylated p53s were associated with MAPK (mitogen-activated protein kinase) and the activation of MAPK kinase could target the transcription factors to anti-virus infection . Thus, our result agreed with Shin and others, who reported infected cells recognized viral replication as a DNA damage stress and elicit the host surveillance mechanism to anti-virus infection . On the other hand, malignant transformation usually takes a long term, where it is important that oncogene E6 integrated in the genome of host and degraded p53 . It was easy to understand, in the present study, we constructed a short transient expression system, the over-expression of E6 can partly mimic the large infection of HPV in the early stage, where the oncogene E6 is not integrated in the genome of host. With over-expressed exotic DNA of E6, the host cells might clean them up through activating apoptosis pathway. This also agreed with authors, who reported compared to the prevalence of HPV infections in the general population, the number of lesions that progress to cancer is very low . The cells infection with high risk HPV might take a self limited process through apoptosis mechanism without progressing to cancer. Thus, the present study gave us an implication that giving the patient correct treatment on the early stage of HPV infection, which will be helpful not to progress to cancer.
In conclusion, the present study provided a new pattern of interaction between HPV-18E6 and p53. That was, GFP-18E6 could transiently induce p53 phosphorylation at three sites, Ser15, Ser20, and Ser392 in both 293T and MCF-7 cells by the activation of ATM and/or Chk2 pathway.
This study was supported by grants from Xi'an Jiaotong University, China.
- Zur Hausen H: Papillomaviruses in human cancer. Cancer. 1987, 59: 1692-1696. 10.1002/1097-0142(19870515)59:10<1692::AID-CNCR2820591003>3.0.CO;2-F.View ArticleGoogle Scholar
- Gumus M, Yumuk PF, Salepci T, Aliustaoglu M, Dane F, Ekenel M, Basaran G, Kaya H, Barisik N, Turhal NS: HPV DNA frequency and subset analysis in human breast cancer patients' normal and tumoral tissue samples. J Exp Clin Cancer Res. 2006, 25: 515-521.Google Scholar
- Kehmeier E, Rühl H, Voland B, Stöppler MC: Cellular steady-state levels of "high risk" but not "low risk" human papillomavirus (HPV) E6 proteins are increased by inhibition of proteasome-dependent degradation independent of their p53- and E6AP-binding capabilities. Virology. 2002, 299: 72-87. 10.1006/viro.2002.1502.View ArticleGoogle Scholar
- Mantovani F, Banks L: The human papillomavirus E6 protein and its contribution to malignant progression. Oncogene. 2001, 20: 7874-7887. 10.1038/sj.onc.1204869.View ArticleGoogle Scholar
- Indinnimeo M, Cicchini C, Stazi A, Giarnieri E, French D, Limiti MR, Ghini C, Vecchione A: Human papillomavirus infection and p53 nuclear overexpression in anal canal carcinoma. J Exp Clin Cancer Res. 1999, 18: 47-52.Google Scholar
- Scheffner M, Werness BA, Huibregtse JM: The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. Cell. 1990, 63: 1129-1136. 10.1016/0092-8674(90)90409-8.View ArticleGoogle Scholar
- Kawamata Y, Mitsuhashi A, Unno Y, Kado S, Shino Y, Uesugi K, Eguchi O, Ishii J, Seki K, Sekiya S, Shirasawa H: HPV 16-E6-mediated degradation of intrinsic p53 is compensated by upregulation of p53 gene expression in normal cervical keratinocytes. Int J Oncol. 2002, 21: 561-567.Google Scholar
- Butz K, Shahabeddin L, Geisen C, Spitkovsky D, Ullmann A, Hoppe-Seyler F: Functional p53 protein in human papillomavirus-positive cancer cells. Oncogene. 1995, 10: 927-936.Google Scholar
- Fouret P, Dabit D, Sibony M, Alili D, Commo F, Saint-Guily JL, Callard P: Expression of p53 protein related to the presence of human papillomavirus infection in precancer lesions of the larynx. Am J Pathol. 1995, 146: 599-604.Google Scholar
- Inoue H, Kondoh G, Sudiro TM, Hakura A: Stability of p53 protein in rat cells transformed by various viral transforming genes. Virology. 1992, 187: 343-347. 10.1016/0042-6822(92)90325-J.View ArticleGoogle Scholar
- Tanaka K, Inoue Y, Hiro J, Yoshiyama S, Toiyama Y, Eguchi T, Miki C, Kusunoki M: Schedule-dependent cytotoxicity of 5-fluorouracil and irinotecan in p53 mutant human colon cancer. J Exp Clin Cancer Res. 2007, 26: 241-251.Google Scholar
- Lu X, Lane DP: Differential induction of transcriptionally active p53 following UV or ionizing radiation: defects in chromosome instability syndromes?. Cell. 1993, 75: 765-778. 10.1016/0092-8674(93)90496-D.View ArticleGoogle Scholar
- Böttger S, Jerszyk E, Low B, Walker C: Genotoxic stress-induced expression of p53 and apoptosis in leukemic clam hemocytes with cytoplasmically sequestered p53. Cancer Res. 2008, 68: 777-782. 10.1158/0008-5472.CAN-06-0968.View ArticleGoogle Scholar
- Lavin MF, Gueven N: The complexity of p53 stabilization and activation. Cell Death Differ. 2006, 13: 941-950. 10.1038/sj.cdd.4401925.View ArticleGoogle Scholar
- Shin YC, Nakamura H, Liang X, Feng P, Chang H, Kowalik TF, Jung JU: Inhibition of the ATM/p53 signal transduction pathway by Kaposi's sarcoma-associated herpesvirus interferon regulatory factor 1. J Virol. 2006, 80: 2257-2266. 10.1128/JVI.80.5.2257-2266.2006.View ArticleGoogle Scholar
- Granja AG, Nogal ML, Hurtado C, Salas J, Salas ML, Carrascosa AL, Revilla Y: Modulation of p53 cellular function and cell death by African swine fever virus. J Virol. 2004, 78: 7165-7174. 10.1128/JVI.78.13.7165-7174.2004.View ArticleGoogle Scholar
- Li L, Guo L, Tao Y, Zhou S, Wang Z, Luo W, Hu D, Li Z, Xiao L, Tang M, Yi W, Tsao SW, Cao Y: Latent membrane protein 1 of Epstein-Barr virus regulates p53 phosphorylation through MAP kinases. Cancer Lett. 2007, 255: 219-231. 10.1016/j.canlet.2007.04.014.View ArticleGoogle Scholar
- Kurz EU, Lees-Miller SP: DNA damage-induced activation of ATM and ATM-dependent signaling pathways. DNA Repair. 2004, 3: 889-900. 10.1016/j.dnarep.2004.03.029.View ArticleGoogle Scholar
- Banin S, Moyal L, Shieh S, Taya Y, Anderson CW, Chessa L, Smorodinsky NI, Prives C, Reiss Y, Shiloh Y, Ziv Y: Enhanced phosphorylation of p53 by ATM in response to DNA damage. Science. 1998, 281: 1674-1677. 10.1126/science.281.5383.1674.View ArticleGoogle Scholar
- Matsuoka S, Rotman G, Ogawa A, Shiloh Y, Tamai K, Elledge SJ: Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro. Proc Natl Acad Sci USA. 2000, 97: 10389-10394. 10.1073/pnas.190030497.View ArticleGoogle Scholar
- Li X, Coffino P: High-Risk human papillomavirus E6 protein has two distinct binding sites within p53, of which only one determines degradation. J Virol. 1996, 70: 4509-4516.Google Scholar
- Giannakakou P, Nakano M, Nicolaou KC: Enhanced microtubule-dependent trafficking and p53 nuclear accumulation by suppression of microtubule dynamics. Proc Natl Acad Sci USA. 2002, 99: 10855-10860. 10.1073/pnas.132275599.View ArticleGoogle Scholar
- Rahman-Roblick R, Hellman U, Becker S: Proteomic identification of p53-dependent protein phosphorylation. Oncogene. 2008, 27: 4854-4859. 10.1038/onc.2008.124.View ArticleGoogle Scholar
- Kastan MB, Onyekwere O, Sidransky D, Vogelstein B, Craig RW: Participation of p53 protein in the cellular response to DNA damage. Cancer Res. 1991, 51: 6304-6311.Google Scholar
- Kessis TD, Slebos RJ, Nelson WG, Kastan MB, Plunkett BS, Han SM, Lorincz AT, Hedrick L, Cho KR: Human papillomavirus 16 E6 expression disrupts the p53-mediated cellular response to DNA damage. Proc Natl Acad Sci USA. 1993, 90: 3988-3992. 10.1073/pnas.90.9.3988.View ArticleGoogle Scholar
- Cinti C, Claudio PP, Luca AD, Cuccurese M, Howard CM, D'Esposito M, Paggi MG, Sala DL, Azzoni L, Halazonetis TD, Giordano A, Maraldi NM: A serine 37 mutation associated with two missense mutations at highly conserved regions of p53 affect pro-apoptotic genes expression in a T-lymphoblastoid drug resistant cell line. Oncogene. 2000, 19: 5098-5105. 10.1038/sj.onc.1203848.View ArticleGoogle Scholar
- Damia G, Filiberti L, Vikhanskaya F, Carrassa L, Taya Y, D'incalci M, Broggini M: Cisplatinum and taxol induce different patterns of p53 phosphorylation. Neoplasia. 2001, 3: 10-16. 10.1038/sj.neo.7900122.View ArticleGoogle Scholar
- Pabla N, Huang S, Mi QS, Daniel R, Dong Z: ATR-Chk2 signaling in p53 activation and DNA damage response during cisplatin-induced apoptosis. J Biol Chem. 2008, 283: 6572-6583. 10.1074/jbc.M707568200.View ArticleGoogle Scholar
- Craig AL, Burch L, Vojtesek B, Mikutowska J, Thompson A, Hupp TR: Novel phosphorylation sites of human tumour suppressor protein p53 at Ser20 and Thr18 that disrupt the binding of mdm2 (mouse double minute 2) protein are modified in human cancers. Biochem J. 1999, 342: 133-141. 10.1042/0264-6021:3420133.Google Scholar
- Saito S, Yamaguchi H, Higashimoto Y, Chao C, Xu Y, Fornace AJ, Appella E, Anderson CW: Phosphorylation site interdependence of human p53 post-translational modifications in response to stress. J Biol Chem. 2003, 278: 37536-37544. 10.1074/jbc.M305135200.View ArticleGoogle Scholar
- Sayed M, Pelech S, Wong C, Marotta A, Salh B: Protein kinase CK2 is involved in G2 arrest and apoptosis following spindle damage in epithelial cells. Oncogene. 2001, 20: 6994-7005. 10.1038/sj.onc.1204894.View ArticleGoogle Scholar
- Wallace M, Coates PJ, Wright EG, Ball KL: Differential posttranslational modification of the tumour suppressor proteins Rb and p53 modulate the rates of radiation-induced apoptosis in vivo. Oncogene. 2001, 20: 3597-3608. 10.1038/sj.onc.1204496.View ArticleGoogle Scholar
- Hao M, Lowy AM, Kapoor M, Deffie A, Liu G, Lozano G: Mutation of phosphoserine 389 affects p53 function in vivo. J Biol Chem. 1996, 271: 29380-29385. 10.1074/jbc.271.46.29380.View ArticleGoogle Scholar
- Werness BA, Levine AJ, Howley PM: Association of human papillomavirus types 16 and 18 E6 proteins with p53. Science. 1990, 248: 76-79. 10.1126/science.2157286.View ArticleGoogle Scholar
- Doorbar J: The papillomavirus life cycle. J Clin Virol. 2005, 32: S7-15. 10.1016/j.jcv.2004.12.006.View ArticleGoogle Scholar
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