Figure 2

NSBP1 knockdown decreases the proliferation of ccRCC cells. (A), MTT assay showing that NSBP1 knockdown significantly reduced the proliferation of ccRCC cells over the 96 h period. (B), Annexin V-PE/7-AAD staining in FCM assays showing the ratio of apoptosis in different 786-O cells. (Data were presented as mean ± SEM, n = 3). (C), Representative blots demonstrating the reduced protein level of NSBP1 in NSBP1 siRNA transfected 786-O cells compared to scramble siRNA transfected cells. In addition, Bax protein level was increased and CyclinB1 and Bcl-2 levels were decreased in NSBP1 siRNA transfected 786-O cells compared to scramble siRNA transfected cells.