An integrated analysis of miRNA and gene copy numbers in xenografts of Ewing's sarcoma
- Neda Mosakhani†1,
- Mohamed Guled1,
- Gayle Leen2,
- Silvia Calabuig-Fariñas3,
- Tarja Niini1,
- Isidro Machado3,
- Suvi Savola1,
- Katia Scotlandi4,
- José Antonio López-Guerrero5,
- Antonio Llombart-Bosch3 and
- Sakari Knuutila1Email author
© Mosakhani et al; licensee BioMed Central Ltd. 2012
Received: 18 January 2012
Accepted: 20 March 2012
Published: 20 March 2012
Xenografts have been shown to provide a suitable source of tumor tissue for molecular analysis in the absence of primary tumor material. We utilized ES xenograft series for integrated microarray analyses to identify novel biomarkers.
Microarray technology (array comparative genomic hybridization (aCGH) and micro RNA arrays) was used to screen and identify copy number changes and differentially expressed miRNAs of 34 and 14 passages, respectively. Incubated cells used for xenografting (Passage 0) were considered to represent the primary tumor. Four important differentially expressed miRNAs (miR-31, miR-31*, miR-145, miR-106) were selected for further validation by real time polymerase chain reaction (RT-PCR). Integrated analysis of aCGH and miRNA data was performed on 14 xenograft passages by bioinformatic methods.
The most frequent losses and gains of DNA copy number were detected at 9p21.3, 16q and at 8, 15, 17q21.32-qter, 1q21.1-qter, respectively. The presence of these alterations was consistent in all tumor passages. aCGH profiles of xenograft passages of each series resembled their corresponding primary tumors (passage 0). MiR-21, miR-31, miR-31*, miR-106b, miR-145, miR-150*, miR-371-5p, miR-557 and miR-598 showed recurrently altered expression. These miRNAS were predicted to regulate many ES-associated genes, such as genes of the IGF1 pathway, EWSR1, FLI1 and their fusion gene (EWS-FLI1). Twenty differentially expressed miRNAs were pinpointed in regions carrying altered copy numbers.
In the present study, ES xenografts were successfully applied for integrated microarray analyses. Our findings showed expression changes of miRNAs that were predicted to regulate many ES associated genes, such as IGF1 pathway genes, FLI1, EWSR1, and the EWS-FLI1 fusion genes.
KeywordsEwing's sarcoma xenograft MicroRNA Copy number Microarray
Due to active international collaboration in the study of rare tumors, such as in Ewing's sarcoma (ES), a great body of tumor-related molecular biomarkers have already been mined by novel array technologies and the clinical significance of some of the biomarkers has been established . A limiting factor for the research of rare bone tumors has been the limited availability of research material derived from patients. Therefore, xenografts, tumors grown from human tumor cells and implanted in immunodeficient animals, are a viable option that is widely used for in vivo models [2, 3]. Xenografted tumors are enriched for neoplastic cells with the minimal contaminating mouse stromal tissue, a property that makes them suitable for molecular analysis . Several studies have shown that xenograft tumors may provide an accurate reflection of tumor biology [5–9].
MicroRNAs (miRNAs) are small, single-stranded non-coding endogenous RNAs, consisting of 20-23 nucleotides, typically acting as post-transcriptional repressors [10, 11]. Despite the fact that miRNAs have been implicated in more than 70 diseases, they have never been investigated, to our knowledge, in the tumor/xenograft setting  (http://cmbi.bjmu.edu.cn/hmdd).
Here, we have performed miRNA- and comparative genomic hybridization (CGH) array analyses on a series of ES xenografts to investigate differential miRNA expression and genomic DNA copy number changes, which are potentially involved in the tumorigenesis of ES. These results have been assessed to identify whether copy number alterations influence miRNA expression, since DNA copy number abnormalities can have a direct impact on the miRNA expression levels . Multiple xenograft passages from each primary tumor were tested to enhance the statistical power of the study.
Ewing sarcoma xenograft series, 6, originating from five patients
Case No. (Nude)
1*, 2*, 4, 7*, 11, 14*
0, 1, 4, 11, 15, 22
0, 4, 11*, 15*, 18, 21*
0, 1, 5*, 11, 17, 25*
430 (PRI) (230)
0, 1*, 4, 9, 19*
430 (MET) (248)
1*, 4, 14*, 21, 30*
The stem cells were obtained from human primary bone marrow-derived mesenchymal stem cells after informed patient consent; precisely, from bone marrow aspirates (iliac crest) of patients undergoing hip replacement surgery. Nucleated cells were placed in modified alpha-MEM media (Li StarFish) containing 20% fetal bovine serum (Cambrex Bioscience), 100 units/mL penicillin (Life Technologies), 100 mg/mL streptomycin (Life Technologies), and 2 mmol/L glutamax (Life Technologies). Confluent cells were harvested by trypsin/EDTA and seeded at 1:3 density.
Male nude mice were purchased from IFFA-CREDO (Lyon, France) and kept under specific pathogen-free conditions throughout the experiment (with vinyl isolates plus sterilized food, water, cage, and bedding). The specimens for xenografting were obtained from the surgery of original tumors and placed in the culture medium (RPMI 1640) with antibiotics at 37°C until the transplantation (usually less than 2 hours after the surgery). Various fragments of the non-necrotic tumor, about 3-5 mm in size, were xenografted into the subcutaneous tissue of the backs of nude mice. The cells from this first implantation are denoted as passage 0 cells and are considered to represent primary tumors. After allowing the growth to approximately 2-3 cm, the subsequent tumor transfers were performed following the same procedures as in the initial xenotransplant and always under highly sterile conditions. In each passage, sufficient amount of material was obtained for the histopathology analysis (Formalin-fixed paraffin-embedded tissue blocks from which tissue microarrays were constructed), the touch preparations, the electron microscopy, the tissue culture, and frozen tissue. All the experimentation involving laboratory animals was approved by the Institutional Animal Care of Valencia University and the Local Government and was performed in accordance with the national legislation of Spain.
The ploidy analysis was not seen necessary to be performed as both histopathological and copy number analysis did not provide any evidence of polyploidy.
Nucleic acid isolation
Genomic DNA from the 34 passages (Table 1) was extracted by the standard phenol-chloroform method. Reference DNAs, male and female, were extracted from the pooled blood samples (4 individuals each) obtained from the Blood Service, Red Cross, Finland. The Qiagen's miRNeasy Mini Kit (Qiagen, Valencia, CA, USA) was used to extract total RNA, including miRNA, according to the manufacturer's instructions. The Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA) was used for quantification of DNA and RNA. The quality of DNA was checked by gel electrophoresis, while for the quality of total RNA and miRNA, the Agilent bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) was applied.
Array CGH hybridization, scanning and data analysis
The Agilent Human Genome CGH 4x44A oligo microarrays (Agilent Technologies, Santa Clara, CA, USA) containing ~44,000 oligonucleotide probes were used. Digestion, labeling, and hybridization of DNA were done according to the manufacturer's instructions (Agilent protocol version 2.0). Briefly, the same amounts (1.5 μg) of patient DNA and gender matched reference DNA were digested. The digested DNAs were labelled by random priming with Cy3-dUTP (reference DNA) and Cy5-dUTP (patient DNA) by use of the Agilent Labelling Kit, after which the labelled DNAs were purified. Next, differentially labelled patient and reference DNAs were combined and hybridized to Agilent Human Genome CGH 4x44A microarrays at 65°C for 24 hours. The hybridized arrays were washed and, then, scanned with the Agilent's microarray scanner (G2565BA, Agilent Technologies, Santa Clara, CA, USA). The raw data was extracted from the array images by the Agilent's Feature Extraction Software (version 8.1). The data was analyzed with the Agilent CGH Analytics software (version 3.4) using ADM-2 algorithm (threshold 6.0) with 1.0 Mb window size.
MicroRNA hybridization, scanning and data processing
We used the Agilent's miRNA microarray system (V3), containing 866 human and 89 human viral miRNAs catalogued in the Sanger miRNA database v12 (Agilent Technologies, Santa Clara, CA, USA). Labelling and hybridization of RNA samples was performed with the Agilent's miRNA Complete Labelling and Hyb Kit. Accordingly, 100 ng of total RNA were treated with Calf Intestine Phosphatase for 30 min at 37°C; 100% DMSO was used for denaturation at 100°C for 5 min, after which the samples were immediately transferred into an ice water bath to prevent reannealing. Next, samples were labelled with cyanine 3-pCp by incubating with T4 RNA ligase for 2 hours at 16°C. After the labeling reaction, the samples were vacuum dried at medium heat and re-suspended in nuclease-free water. Next, samples were hybridized to the microarrays in the Agilent SureHyb chambers (Agilent Technologies) for 20 hours at 55°C, after which the microarrays were washed with the manufacturer's washing buffers. The arrays were scanned using the Agilent's scanner and the raw data were preprocessed with the Agilent's Feature Extraction Software with default parameters. Details of the miRNA preprocessing protocol are provided by the manufacturer.
Statistical analysis was carried out with the GeneSpring GX analysis software (version 10) and the R statistical programming language (http://www.r-project.org). The data were preprocessed by adding offsets and carrying out normalization between all the arrays by the quantile method, and taking log2 transformation. The data were filtered by removing control miRNAs and the miRNAs that were not detected across any of the samples. Detection calls were provided by the Agilent's Feature Extraction Software. MiRNAs with less than the threshold of the ratio of total gene signal/total gene error under three were considered to be undetected. The detected miRNAs were regarded as present in the measured sample. We also removed miRNAs based on their expression: for each miRNA, its expression had to exceed in at least one array (negative control miRNAs' expression) + 1.5× standard deviation (negative control miRNAs' expression). We examined the detection calls for each sample to determine which miRNAs were expressed or not expressed. To find differentially expressed miRNAs between controls and xenograft samples, we performed moderated t-tests for each miRNA to find significant differences in miRNA expression between the two groups and, then, calculated the most significant up-regulated and downregulated miRNAs (adjusted p value [q value] < 0.05, Bonferroni correction to correct for multiple testing).
Real-time polymerase chain reaction (real-time RT-PCR) analysis
To validate the selected miRNA expression levels in ES samples compared to control samples, RT-PCR analysis was applied. The miScript Reverse Transcription Kit (Qiagen, Valencia, CA) served for reverse transcription of RNA, according to manufacturer's guidelines. QRT-PCR was performed on a Light-cycler, software v.3.5 (Roche Applied Science, Mannheim, Germany) by the SYBR Green miScript PCR system (Qiagen). Each reaction was performed in a 20-μl volume with 5 ng template cDNA. The primers for amplification of selected miRNAs and snRNA U6 were purchased from the Qiagen. The experiments were performed in duplicate for each RNA sample, and every run included a control without template. The U6 primer assay (Qiagen) served as an endogenous control for normalization. The relative quantification (RQ) for each miRNA, compared with U6 was calculated using equation 2-ΔΔCt.
Relationship between miRNA and CGH data
We investigated whether any association existed between miRNA expression changes and gain/loss of genomic regions. We mapped each miRNA to its chromosomal band location, which was retrieved from the Ensembl, using the biomaRt package, and the mirBase database. For each miRNA, we counted the number of xenograft samples (out of 14) in which there was loss, gain, or no change in copy number for the corresponding chromosomal band. Possible associations were determined by counting the number of samples showing miRNA over-expressed/genomic gain and miRNA under-expressed/genomic loss. We also counted the number of control samples (out of 2) in which the miRNA was detected.
Predicted targets of differentially expressed miRNAs
After having acquired the differentially expressed miRNAs, we used the miRBase target prediction database (http://microrna.sanger.ac.uk), TargetScan (http://www.targetscan.org), and miRanda (http://www.microRNA.org) for evaluation of the predicted mRNA targets. The list of predicted mRNA targets was screened for the genes known to be functionally relevant in ES and predicted at least by one of the algorithms.
Copy number alterations in xenografts
The copy number changes present in all the passages of each xenograft series
Case No. (Nude)
Array CGH results
-2q35-q37.3 (uncontinuous), + 8, +15, +17q21.32-qter
-1q24.3-q25.2, - 3p12.3-p24.3, -9p21.3
+1q, -16q, -9p21.3
430 (PRI) (230)
430 (MET) (248)
Copy number changes present in only part of the passages of each xenograft series
Array CGH result
15- 2, 4, 7, 11, 14
15- 1, 2, 4, 7
53- 11, 15,18, 21
199- 5, 11, 17, 25
230- 1, 4, 9, 19
248- 1, 4
248- 14, 21, 30
-1p36.21-pter, -1p13.3-p31.1, -5q,
248- 21, 30
MicroRNA alterations in xenografts
The 46 miRNAs detected in all xenografts samples, while absent from all control samples.
MiRNAs expressed in xenograft passages of A) Case 430 primary tumor while absent in lung metastasis, 12 miRNAs, B) Case 430 lung metastasis while absent in primary tumor, 18 miRNAs and C) Case 430 primary tumors and control, while absent in lung metastasis, 11 miRNAs
miRNAs expressed in
A) Xenograft passages from Primary tumor (12 miRNAs)
B) Xenograft passages from lung metastasis (18 miRNAs)
C) Control and xenograft passages from Primary tumor (11 miRNAs)
Validation of differentially expressed miRNAs by qRT-PCR
QRT-PCR was used to validate the expression levels of four selected miRNAs that showed altered expression in the miRNA microarray results. The selection of miRNAs for further validation was based on the expression level of miRNA microarray results and on the level of representation in the expression categories observed (i.e. exclusively expressed, significantly under-expressed and significantly over-expressed). The miR-31 and miR-31* were exclusively expressed in control samples and absent in xenograft passages, while miR-106b was significantly over-expressed and miR-145 significantly under-expressed, respectively, in xenograft samples compared to control samples.
As for the validation results by qRT-PCR, the expression levels of miR-31, miR-31* and miR-145 were under-expressed in the xenograft samples compared to the control samples (relative expression 0.00062, 0.00809 and 0.09111, respectively). These results are consistent with the miRNA microarray results. Similarly, the over-expression of miR-106b in xenograft samples seen in miRNA microarray was confirmed by qRT-PCR results showing relative expression level of 87.7.
Relationship between miRNAs and copy number alterations
A joint analysis of the aCGH data and miRNA data for the 14 xenograft passages, which were common to both studies, was performed by looking for miRNAs whose expression was correlated with a change (loss/gain) at their chromosomal location. Three criteria were used to determine the miRNAs of greatest interest: (i) differentially expressed miRNAs in all 14 xenograft passages, (ii) altered miRNAs whose chromosomal locations were affected by the same copy number changes in most of the passages, and (iii) miRNAs fulfilling both previous criteria.
Altered miRNAs in regions of copy number changes
miRNA in copy number gain
miRNA in copy number loss
Number of samples
Number of samples
in gain region
in loss region
Here we have sought to identify differentially expressed miRNAs in ES xenografts and to investigate the underlying molecular changes by integration of these results with aCGH analysis of the same samples.
MiRNA expression profile of ES xenografts
Xenografts displayed 60 differentially expressed miRNAs that distinguished them from control samples (Human mesenchymal stem cells). Of these, 46 miRNAs were exclusively expressed in xenografts while 2 (miR-31 and miR-31*) miRNAs were exclusively expressed in controls. The remaining 5 miRNAs (miR-106b, miR-93, miR-181b, miR-101, miR-30b) were significantly over-expressed while 6 miRNAs (miR-145, miR-193a-3p, miR-100, miR-22, miR-21, miR-574-3p) were significantly under-expressed in xenografts. The expression profiles of 4 miRNAs (miR-31, miR-31*, miR-106b, miR-145) were confirmed by RT-PCR.
To evaluate the potential role of the differentially expressed miRNAs, three databases were searched for the known ES-associated genes targeted by these miRNAs, by applying target prediction algorithms. The targets included EWSR1 (GeneID: 2130), FLI1 (GeneID: 2313), SOX2 (GeneID: 6657), p53 (GeneID: 7157), IGFBP3 (GeneID: 3486), IGF1 (GeneID: 3479) and IGF1R (GeneID: 3480). The differential expression of the miRNAs regulating these genes may play a role in the tumorigenesis and tumor progression of ES.
Interestingly, miR-150, which targets the tumor suppressor gene TP53, was expressed in all xenograft samples but in none of the control samples. This is in accordance with the study of Fabbri and colleagues  who have included TSGs in their investigation of likely over-expressed miRNA target genes. In addition, one of our xenograft series (Case number 451) showed losses at 17p, containing TP53, that appeared in later passages. Previous ES studies have shown that, despite the low frequency of mutations in TP53, an alteration of TP53, in conjunction with the deletion of CDKN2A, is associated with a poor clinical outcome [23, 24]. Moreover, the homozygous deletion of this gene has been reported in a small subset of ES patients [25, 26].
The IGF-1 pathway, whose genes IGF1R, IGF-1 and IGFBP-3 are among the target genes of the differentially expressed miRNAs, plays a critical role in cancer development, including ES [26–28]. IGF1R is targeted by miR-145 and miR-31*, and previous studies have shownIGF1R to be a direct target of miR-145  as well as to be over-expressed in Ewing tumors [27, 28]. As for IGF-1, it is the target of 11 miRNAs including miR-21, miR-31, miR-145, miR-150, miR-194, miR-215, miR-421, miR-486-5p, 548c-5p, and miR-873. Interestingly, IGFBP3, which is among the target genes of miR-150*, was, in our study, expressed in all xenografts but not in control samples. IGFBP-3, which is a major regulator of cell proliferation and apoptosis, inhibits the interaction of IGF-1 with its receptor (IGF1R) [30–33]. Indeed, it has been reported that high IGF-1 and low IGFBP-3 levels in serum increase the risk of cancer . IGFBP3 is strongly down-regulated by the EWS/FLI-1 fusion gene , which is able to induce expression of embryonic stem cell gene SOX2. Consequently, SOX2 participates in ES cell proliferation and tumorigenesis and might play a central role in ES pathogenesis . As for our study, SOX2 was among the target genes of miRNA-21 that showed under-expression in xenografts. Another under-expressed miRNA, miR-145, was previously found to target FLI1 and its increased expression leads to a decreased migration of microvascular cells in response to the growth factor gradients in vitro .
Finally, miR-106b targets EWSR1, which undergoes a chromosomal translocation to produce the EWS-FLI fusion gene in a majority of ES cases, where it is commonly considered to trigger the condition. The action of miR-106b is, thus, likely to only impact on the original/unmodified locus for EWSRI since the EWS-FLI lacks the 3' portion of EWSR1. Further studies would, naturally, be required to confirm this hypothesis.
The alteration of 41 miRNAs was observed in xenograft passages derived from lung metastatic, which may play a crucial role in triggering tumor metastasis. Eight of these miRNAs, all located at the 14q32 imprinted domain (miR-154*, miR-337-3P, miR-369-5p, miR-409-5p, miR-411, miR-485-3p, miR-487a, miR-770-5p) were not expressed in metastasis xenografts but in control samples, thus suggesting a tumor suppressor function. Interestingly, gastrointestinal stromal tumors (GISTs) have displayed 44 expressed miRNAs originatingfrom the 14q32 chromosomal region, for which the low expression of miRNAs was related to tumor progression . A report by Saito and colleagues  suggests that miRNAs located in this region function as tumor repressor genes and changes in the methylation status of their promoters could trigger cancer development. This evidence suggests that the miRNAs identified in our study may act as tumor repressors and their absence could increase the risk of metastasis and tumor progression in ES.
Copy number aberrations in ES xenografts
The most recurrent copy number alterations detected in our CGH analysis (gains at chromosome 8, 1q and losses at 9p21.3 and 16q) are in agreement with other findings on ES patients [1, 39–46]. The crucial role of these changes, gains in 1q, 8 and losses of 9p21.3 (including loss of CDKN2A) and 16q, has been clarified by notable tumor development and adverse clinical outcome [42, 47, 48]. These copy number changes were seen throughout the whole xenograft series. In all passages of lung metastasis, losses were observed at 1p36.12-pter/1p36.21-pter. Of note, deletion of this site (1p36) has been found to be related to a poor clinical outcome in ES[43, 47]. The loss of 1p36.12-pter in the first two passages originating from lung metastasis (1 and 4) changed to loss of 1p36.21-pter in the last three passages (14, 21 and 30). The lung metastasis xenografts showed 9 copy number changes, whereas only 3 of these aberrations were observable in the xenograft passages from its primary tumor. Likewise in many tumors during the disease progression, the increase of genomic instability is also seen here. This instability most probably explains the variation of the size of 1p deletion. The fact that the terminal part is retained in the deletion emphasizes the importance of 1p36.21-pter region in the selection and in the disease progression.
Somatic mosaicism/heterogeneity occurs commonly in tumors and plays an important role in the progression of the tumor and, thereby, can also explain why some xenograft passages show copy number changes and others do not.
Integration of miRNA expression profiles and DNA copy number changes
DNA copy number abnormalities can have a direct impact on miRNA expression . In the current study, 20 differentially expressed miRNAs were located in the copy number altered regions. These findings are in accordance with Calin et al. (2004) who observed that half of the miRNAs are located in cancer-associated regions of chromosomes as well as in genomic regions frequently amplified or lost in cancer . The target genes for many of the changes are still unknown and, therefore, miRNAs could well be considered to be the drivers of the underlying changes.
MiR-31 and miR-31*, targeting IGF1 and IGF1R, are located at the frequently deleted region of 9p21.3, containing the CDKN2A gene, which was frequently lost in our samples. Under-expression of miR-31 or deletion of the miR-31 genomic locus is also found in human breast cancers. This miRNA regulates metastasis by opposing local invasion and metastatic colonization in this cancer [50–52]. Chromosome 1 gain is a frequent gain that contains the whole chromosome and seems to be poor prognostic sign . Interestingly, in our study five overexpressed miRNAs (miR-765, miR-135b, miR29c, miR-215, and miR-557) (Table 6) were associated to 1q gain. These candidate miRNAs have an important role and could explain the underlying mechanism in ES. Nevertheless, functional validations of the predicted target genes are needed to better understand their role in the ES tumorgnesis.
The current study provides new information about the miRNA expression and its relationship with the associated genomic copy number changes in ES xenografts. Our findings suggest that miRNAs play a role in the molecular pathogenesis and tumorigenesis of ES by regulating important genes in the IGF1 pathway as well as the genes FLI1, EWSR1, and the EWS-FLI1 fusion gene. In addition, integration of the data for gene copy number changes and miRNA profiles demonstrated some cases where the differential expression of miRNAs was the result of copy number alteration of the miRNA genomic locus. Moreover, our study showed that the xenografts can reflect well the genomic pattern of their original tumor.
The study was supported by grants from European Commission (EuroBoNeT Project LSHC-CT-2006-018814) and the special state subsidy research funds appropriated to the Helsinki and Uusimaa Hospital District (HUS EVO).
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