A novel oncolytic viral therapy and imaging technique for gastric cancer using a genetically engineered vaccinia virus carrying the human sodium iodide symporter
© Jun et al.; licensee BioMed Central Ltd. 2014
Received: 13 October 2013
Accepted: 11 December 2013
Published: 2 January 2014
Gastric cancers have poor overall survival despite recent advancements in early detection methods, endoscopic resection techniques, and chemotherapy treatments. Vaccinia viral therapy has had promising therapeutic potential for various cancers and has a great safety profile. We investigated the therapeutic efficacy of a novel genetically-engineered vaccinia virus carrying the human sodium iodide symporter (hNIS) gene, GLV-1 h153, on gastric cancers and its potential utility for imaging with 99mTc pertechnetate scintigraphy and 124I positron emission tomography (PET).
GLV-1 h153 was tested against five human gastric cancer cell lines using cytotoxicity and standard viral plaque assays. In vivo, subcutaneous flank tumors were generated in nude mice with human gastric cancer cells, MKN-74. Tumors were subsequently injected with either GLV-1 h153 or PBS and followed for tumor growth. 99mTc pertechnetate scintigraphy and 124I microPET imaging were performed.
GFP expression, a surrogate for viral infectivity, confirmed viral infection by 24 hours. At a multiplicity of infection (MOI) of 1, GLV-1 h153 achieved > 90% cytotoxicity in MNK-74, OCUM-2MD3, and AGS over 9 days, and >70% cytotoxicity in MNK- 45 and TMK-1. In vivo, GLV-1 h153 was effective in treating xenografts (p < 0.001) after 2 weeks of treatment. GLV-1 h153-infected tumors were readily imaged by 99mTc pertechnetate scintigraphy and 124I microPET imaging 2 days after treatment.
GLV-1 h153 is an effective oncolytic virus expressing the hNIS protein that can efficiently regress gastric tumors and allow deep-tissue imaging. These data encourages its continued investigation in clinical settings.
KeywordsOncolytic viral therapy GLV-1 h153 Gastric cancer Human sodium iodide symporter (hNIS)
Gastric cancer is one of the most prevalent malignant tumors, especially in Asia . Although early detection methods, development of endoscopic or surgical resection, and more effective chemotherapies have improved the overall survival in patients with gastric cancer, the prognosis of patients with advanced gastric cancer is still poor [2–4]. Most conventional chemotherapy treatments have demonstrated moderate efficiency. One possible explanation for the resistance of gastric cancer to conventional therapy might be its non-susceptibility to apoptosis . However, oncolytic viruses have great therapeutic effects against cancer cells which express high levels of ribonucleotide reductase, DNA-repair enzymes, and are thus resistant to apoptosis [6, 7]. Many of these characteristics which make gastric cancer cells resistant to chemotherapy, make them susceptible to oncolytic viral therapy. Thus, gene therapy using oncolytic virus offers an attractive alternative for the treatment of gastric cancer .
Oncolytic viral therapy has been studied over the past century and shown success in preclinical and clinical testing as a novel cancer treatment modality . Vaccinia virus (VACV) strains are particularly attractive as potential antitumor agents, as they can incorporate large amounts of foreign DNA without reducing their replication efficiency. Moreover, VACV has shown a great safety profile in humans [10–12]. Lastly, in addition to its therapeutic potential, VACV has also been used as a noninvasive imaging technique allowing clinicians to track therapeutic gene delivery in the body [10, 13].
In this publication, we examined the therapeutic potential of a novel VACV expressing the human sodium iodide symporter (hNIS), GLV-1 h153, against gastric cancers in vitro and in vivo, and tested its potential as an imaging tool.
Materials and methods
Human gastric cancer AGS cells (a gastric adenocarcinoma epithelial cell line) were obtained from American Type Culture Collection (ATCC; Manassas, VA) and were cultured in Ham’s F-12 K Medium. Human OCUM-2MD3 cells were a gift from Dr. Masakazu Yashiro (Osaka City University Medical School, Japan) and were grown in Dulbecco’s Modified Eagle’s Medium (DMEM). MKN-74 and TMK-1 cells were provided by Dr. T. Suzuki (Fukushima Medical College, Japan) and were cultured in Roswell Park Memorial Institute (RPMI). MKN-45 was obtained as a gift from Dr. Yutaka Yonemura (Kanazawa University, Japan) and was maintained in RPMI. African green monkey kidney fibroblast (Cercopithecus aethiops; CV-1) cells used for viral plaque assays were purchased from ATCC (Manassas, VA) and grown in the Minimum Essential Medium (MEM). All media were supplemented with 10% FBS, 1% penicillin, and 1% streptomycin.
GLV-1 h153 is a replication-competent, recombinant vaccinia virus derived from its parental strain, GLV-1 h68, via homologous recombination. It contains four inserted cassettes encoding Renilla Aequorea luciferase- green fluorescent protein (RUC-GFP) fusion protein, a reversely inserted human transferrin receptor (rTfr), β-galactosidase, and human sodium iodide symporter (hNIS) into the F14.5, J2R (encoding thymidine kinase), and A56R (encoding hemagglutinin) loci of the viral genome.GLV-1 h153 was provided by Genelux Corporation (R&D facility in San Diego, CA, USA).
4 × 104 cells per well of each cell line were plated in 12-well plates and incubated in a 5% CO2 humidified incubator at 37°C overnight. GLV-1 h153 was added to each well at varying Multiplicity of Infection (MOIs) of 0.01, 0.1, and 1.0. Viral cytotoxicity was tested using a lactate dehydrogenase (LDH) assay daily. Cells were washed with PBS once, and then lysed with 1.35% Triton X-100 (Sigma, St. Louis, MO). The intracellular LDH release following lysis was subsequently measured with CytoTox 96® (Promega, Madison, WI) on a spectrophotometer (EL321e, Bio- Tek Instruments) at 490 nm. Results are expressed as the percentage of surviving cells, which were calculated as the LDH release of infected samples compared to uninfected control. All conditions were tested in triplicate.
Viral replication assay
Supernatants from each infected well were collected daily and immediately frozen at −80°C. Serial dilutions of all supernatant samples were made to perform standard viral plaque assays on confluent CV-1 cells. All samples were measured in triplicates.
In vivomurine flank tumor therapy
All animal experiments were performed under approved protocols and in accordance with ethical guidelines of the Institutional Animal Care and Use Committee (IACUC) at Memorial Sloan-Kettering Cancer Center (MSKCC). MKN-74 xenografts were established in 6- to 8-week-old female nude mice (NCI:Hsd:Athymic Nude-nu, Harlan) by subcutaneously injecting 5 × 106 MKN-74 cells into the right flank. Tumor growth was recorded twice a week using a digital caliber and tumor volume was calculated using the equation, a × b2 × 0.5, in which a and b are the largest and smallest diameters, respectively. When tumors reached a diameter of approximately 6–8 mm in 10 days, animals were grouped into control and treatment groups with equitable tumor sizes. A single dose of 2 × 106 plaque-forming units (PFUs) of GLV-1 h153 in 100 μL PBS or 100 μL of PBS as control were injected intratumorally to each designated tumor. Animals were observed daily for any signs of toxicity, and sacrificed when their tumors reached a diameter of approximately 15 mm.
Fluorescent imaging (Maestro)
In vivo GFP images were obtained using the CRi Maestro system (Cambridge Research and Instrumentation, Woburn, MA) using the appropriate filters (excitation = 445–490 nm, emission = 515 nm long-pass filter, acquisition settings = 500–720 in 10 nm). After each image was obtained, it was spectrally unmixed to remove the background fluorescence. Images were quantified using region of interest (ROI) analysis software that is supplied with the Maestro system.
In vivosingle photon emission computed tomography SPECT imaging
Five MKN-74 xenografts were intratumorally injected with 2 × 107 PFUs GLV-1 h153 and 5 with PBS as controls. Two days after infection, 200 μCi of 99mTc pertechnetate was administered via tail vein injection. 99mTc pertechnetate images were obtained over 10 min, 3 hours after radiotracer administration. Imaging was performed using the dual-detector gamma camera sub-system of the X-SPECT small-animal SPECT-CT system (Gamma Medica, Northridge, CA). The X-SPECT γ-camera system was calibrated by imaging a mouse-size (30 mL) cylinder filled with a measured concentration (MBq/mL) of 99mTc using a photopeak energy window of 126 to 154 keV and low-energy high-resolution collimation. The resulting 99mTc images were exported to Interfile and then imported into the ASIPro (Siemens Pre-clinical Solutions, Knoxville, TN) image processing software environment. By ROI analysis, a system calibration factor (in cpm/pixel per MBq/mL) was derived. Animal images were likewise exported to Interfile and then imported into ASIPro and parameterized in terms of the decay-corrected percentage injected dose per gram (%ID/g) based on the foregoing calibration factor, the administered activity, the time after administration of imaging, and the image duration.
In vivoPET imaging
Three MKN-74 xenografts were injected intratumorally with 2 × 107 PFU GLV-1 h153 and two with PBS. Two days after viral injection, 300 μCi of 124I was administered via tail vein injection. One hour after radiotracer administration, 3-dimensional list-mode data were acquired using an energy window of 350 to 700 keV, and a coincidence timing window of 6 nanoseconds. Imaging was performed using a Focus 120 microPET dedicated small animal PET scanner (Concorde Microsystems Inc, Knoxville, TN). These data were sorted into 2-dimensional histograms by Fourier rebinning. The count rates in the reconstructed images were converted to activity concentration (%ID/g) using a system calibration factor (MBq/mL per cps/voxel) derived from imaging of a mouse size phantom filled with a uniform aqueous solution of 18F. Image analysis was performed using ASIPro.
Significant differences between groups were determined using Student’s t test (Excel 2007; Microsoft, Redmond, WA, USA). A p-value < 0.05 was considered significant.
In vivomurine xenografts therapy with GLV-1 h153
In vitro and in vivoGFP expression
Functioning hNIS expression imaged by 99mTc-pertechnetate scintigraphy and 124I PET
Gastric cancer is the fourth most common malignancy and the second most frequent cause of cancer-related death world-wide [1, 14]. Recurrence or distant metastasis is one of the most common complications and often the cause of death . While chemotherapy is a useful adjuvant therapy compared to surgical therapy alone, its therapeutic potential is limited . Most gastric cancers are resistant to currently available chemotherapy regimens. Therefore, novel therapeutic agents are needed to improve outcomes for gastric cancer patients who are not responsive to conventional therapies. Oncolytic viral therapy is a promising approach to cancer treatment that depends on the ability of viruses to infect, replicate within, and lyse a host cell [17, 18]. In this study, we described the cytotoxic effects of GLV-1 h153, a novel recombinant VACV carrying the hNIS gene, on gastric cancer cells in vitro. We further demonstrated that GLV-1 h153-infected gastric cancer xenografts expressed functioning hNIS protein that allowed for non-invasive imaging of the tumor and also efficient tumor regression in vivo.
A variety of viruses have shown oncolytic properties including adenovirus, herpes simplex virus, Newcastle disease virus, vesicular stomatitis virus, and reovirus . Among a variety of oncolytic viral agents, vaccinia virus has several advantages. VACV exclusively replicates in the cytoplasm without using the host’s DNA-synthesis machinery, thereby lowering the risk of integration of the viral genome into the host genome . A large amount of foreign DNA (up to 25 kb) can be incorporated without significantly reducing the viral replication efficiency . Moreover, vaccinia has been proven to have a good safety profile as it has been historically given to millions during the smallpox vaccination. It also demonstrates efficient replication and a broad range of host cell tropisms . Several preclinical studies have shown that systemic injection of recombinant VACV into xenografts resulted in high viral titers in tumors only, indicating tumor-specific colonization [11, 20, 21]. There is a small concern that patients who have received smallpox vaccination in the past have neutralizing antibody against the virus. This could potentially result in compromised treatment efficacy. However, in the blood, complement plays a more important role in inactivating VACV than neutralizing antibodies. We therefore predict that the presence of neutralizing antibodies in patients should not hinder VACV treatment; however, a higher treatment dose might be required.
Genetically engineered VACVs have shown efficacy in the treatment of a wide range of human cancers . GLV-1 h168 has already shown to be an effective diagnostic and therapeutic vector in several human tumor models, including breast tumor, mesothelioma, pancreatic cancers, and squamous cell carcinoma  The hNIS protein, which is an intrinsic membrane glycoprotein with 13 putative transmembrane domains, actively transports both Na+ and I- ions across the cell membrane . Functioning hNIS protein can uptake several commercially available radio-nucleotides, including 123I, 124I, 125I, 131I, 99mTc and 188Re [22, 23]. In this study, GLV-1 h153-mediated expression of hNIS protein in infected MKN-74 xenografts resulted in a localized 99mTc and 124I radiotracer uptake. Our results suggest that hNIS gene expression via viral vector can be used as a non-invasive imaging modality to monitor tumor progression and treatment effects.
A single intratumoral injection of GLV-1 h153 in MKN-74 xenografts exhibited localized intratumoral GFP and hNIS expression. Moreover, there was no evidence of viral spread to any other organs based on GFP imaging, 99mTc scintigraphy, and 124I PET, indicating tumor-specific viral infection and activity. We also demonstrated that GLV-1 h153 is effective and safe in treating gastric tumors in a murine xenograft model. The GLV-1 h153-treated group was continuously followed until day 35 and there was no tumor regrowth (data not shown between day 28 and 35). The control group had to be sacrificed in accordance to our approved animal protocol on day 28. Expressing the hNIS gene in an otherwise non-hNIS-expressing tissue is exciting. It could potentially make use of the well-established radioiodine imaging and therapy in other non-thyroid originated cancers. Several studies have shown promising results in a variety of tumors using radioiodine treatment via tumor-specific expression of the hNIS gene, including medullary thyroid carcinoma , prostate cancer , colon cancer , and breast cancer . Tumor-specific hNIS expression using GLV-1 h153 can maximize localized radioiodine accumulation and minimize non-specific uptake in other organs. Based on our promising results, it would be of significant clinical importance to evaluate the effect of combination therapy of GLV-1 h153 and radioiodine.
This study demonstrates a novel oncolytic VACV engineered to express the hNIS can effectively infect, replicate within, and cause regression of gastric cancer in a murine xenograft model. GFP expression can serve as a surrogate of viral infectivity. In vivo, GLV-1 h153 infected cells can be readily imaged with 99mTc scintigraphy and 124I PET imaging. These data provide further support for future investigation of GLV-1 h153 as a treatment agent and a non-invasive imaging tool in the clinical settings.
Human sodium iodide symporter
American Type Culture Collection
Renilla luciferase-Aequorea green fluorescent protein
Lactate dehydrogenase ()
The Institutional Animal Care and Use Committee
Memorial Sloan-Kettering Cancer Center
Multiplicity of infection
Positron emission tomography
Region of interest
Reverse inserted human transferrin receptor
Single photon emission computed tomography.
Technical services provided by the MSKCC Small-Animal Imaging Core Facility, supported in part by NIH Small-Animal Imaging Research Program (SAIRP) Grant No R24 CA83084 and NIH Center Grant No P30 CA08748, are gratefully acknowledged.
- Parkin DM, Bray F, Ferlay J, Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 2005, 55: 74-108. 10.3322/canjclin.55.2.74.View ArticlePubMed
- Wanebo HJ, Kennedy BJ, Chmiel J, Steele G, Winchester D, Osteen R: Cancer of the stomach. A patient care study by the American College of Surgeons. Ann Surg. 1993, 218: 583-592. 10.1097/00000658-199321850-00002.PubMed CentralView ArticlePubMed
- Nakajima T: Gastric cancer treatment guidelines in Japan. Gastric Cancer. 2002, 5: 1-5.View ArticlePubMed
- Park CH, Song KY, Kim SN: Treatment results for gastric cancer surgery: 12 years' experience at a single institute in Korea. Eur J Surg Oncol. 2008, 34: 36-41. 10.1016/j.ejso.2007.03.004.View ArticlePubMed
- Tsunemitsu Y, Kagawa S, Tokunaga N, Otani S, Umeoka T, Roth JA, Fang B, Tanaka N, Fujiwara T: Molecular therapy for peritoneal dissemination of xenotransplanted human MKN-45 gastric cancer cells with adenovirus mediated Bax gene transfer. Gut. 2004, 53: 554-560. 10.1136/gut.2003.021683.PubMed CentralView ArticlePubMed
- Adusumilli PS, Chan MK, Hezel M, Yu Z, Stiles BM, Chou TC, Rusch VW, Fong Y: Radiation-induced cellular DNA damage repair response enhances viral gene therapy efficacy in the treatment of malignant pleural mesothelioma. Ann Surg Oncol. 2007, 14: 258-269.View ArticlePubMed
- Petrowsky H, Roberts GD, Kooby DA, Burt BM, Bennett JJ, Delman KA, Stanziale SF, Delohery TM, Tong WP, Federoff HJ, Fong Y: Functional interaction between fluorodeoxyuridine-induced cellular alterations and replication of a ribonucleotide reductase-negative herpes simplex virus. J Virol. 2001, 75: 7050-7058. 10.1128/JVI.75.15.7050-7058.2001.PubMed CentralView ArticlePubMed
- Cunningham D, Allum WH, Stenning SP, Thompson JN, Van de Velde CJ, Nicolson M, Scarffe JH, Lofts FJ, Falk SJ, Iveson TJ, et al: Perioperative chemotherapy versus surgery alone for resectable gastroesophageal cancer. N Engl J Med. 2006, 355: 11-20. 10.1056/NEJMoa055531.View ArticlePubMed
- Vaha-Koskela MJ, Heikkila JE, Hinkkanen AE: Oncolytic viruses in cancer therapy. Cancer Lett. 2007, 254: 178-216. 10.1016/j.canlet.2007.02.002.View ArticlePubMed
- Chen N, Zhang Q, Yu YA, Stritzker J, Brader P, Schirbel A, Samnick S, Serganova I, Blasberg R, Fong Y, Szalay AA: A novel recombinant vaccinia virus expressing the human norepinephrine transporter retains oncolytic potential and facilitates deep-tissue imaging. Mol Med. 2009, 15: 144-151.PubMed CentralView ArticlePubMed
- Zhang Q, Yu YA, Wang E, Chen N, Danner RL, Munson PJ, Marincola FM, Szalay AA: Eradication of solid human breast tumors in nude mice with an intravenously injected light-emitting oncolytic vaccinia virus. Cancer Res. 2007, 67: 10038-10046. 10.1158/0008-5472.CAN-07-0146.View ArticlePubMed
- Haddad D, Chen NG, Zhang Q, Chen CH, Yu YA, Gonzalez L, Carpenter SG, Carson J, Au J, Mittra A, et al: Insertion of the human sodium iodide symporter to facilitate deep tissue imaging does not alter oncolytic or replication capability of a novel vaccinia virus. J Transl Med. 2011, 9: 36-10.1186/1479-5876-9-36.PubMed CentralView ArticlePubMed
- Brader P, Kelly KJ, Chen N, Yu YA, Zhang Q, Zanzonico P, Burnazi EM, Ghani RE, Serganova I, Hricak H, et al: Imaging a Genetically Engineered Oncolytic Vaccinia Virus (GLV-1 h99) Using a Human Norepinephrine Transporter Reporter Gene. Clin Cancer Res. 2009, 15: 3791-3801. 10.1158/1078-0432.CCR-08-3236.View ArticlePubMed
- Crew KD, Neugut AI: Epidemiology of gastric cancer. World J Gastroenterol. 2006, 12: 354-362.PubMed CentralPubMed
- Yamada E, Miyaishi S, Nakazato H, Kato K, Kito T, Takagi H, Yasue M, Kato T, Morimoto T, Yamauchi M: The surgical treatment of cancer of the stomach. Int Surg. 1980, 65: 387-399.PubMed
- Khan FA, Shukla AN: Pathogenesis and treatment of gastric carcinoma: "an up-date with brief review". J Cancer Res Ther. 2006, 2: 196-199. 10.4103/0973-1482.29830.View ArticlePubMed
- Liu TC, Kirn D: Gene therapy progress and prospects cancer: oncolytic viruses. Gene Ther. 2008, 15: 877-884. 10.1038/gt.2008.72.View ArticlePubMed
- Shen Y, Nemunaitis J: Fighting cancer with vaccinia virus: teaching new tricks to an old dog. Mol Ther. 2005, 11: 180-195.View ArticlePubMed
- B M: Poxviridae: the Viruses and Their Replication. 2001, Philadelphia: Lippincort Williams & Wilkins, 4
- Puhlmann M, Brown CK, Gnant M, Huang J, Libutti SK, Alexander HR, Bartlett DL: Vaccinia as a vector for tumor-directed gene therapy: biodistribution of a thymidine kinase-deleted mutant. Cancer Gene Ther. 2000, 7: 66-73. 10.1038/sj.cgt.7700075.View ArticlePubMed
- Yu YA, Shabahang S, Timiryasova TM, Zhang Q, Beltz R, Gentschev I, Goebel W, Szalay AA: Visualization of tumors and metastases in live animals with bacteria and vaccinia virus encoding light-emitting proteins. Nat Biotechnol. 2004, 22: 313-320. 10.1038/nbt937.View ArticlePubMed
- Hingorani M, Spitzweg C, Vassaux G, Newbold K, Melcher A, Pandha H, Vile R, Harrington K: The biology of the sodium iodide symporter and its potential for targeted gene delivery. Curr Cancer Drug Targets. 2010, 10: 242-267. 10.2174/156800910791054194.PubMed CentralView ArticlePubMed
- Lee YJ, Chung JK, Shin JH, Kang JH, Jeong JM, Lee DS, Lee MC: In vitro and in vivo properties of a human anaplastic thyroid carcinoma cell line transfected with the sodium iodide symporter gene. Thyroid. 2004, 14: 889-895. 10.1089/thy.2004.14.889.View ArticlePubMed
- Cengic N, Baker CH, Schutz M, Goke B, Morris JC, Spitzweg C: A novel therapeutic strategy for medullary thyroid cancer based on radioiodine therapy following tissue-specific sodium iodide symporter gene expression. J Clin Endocrinol Metab. 2005, 90: 4457-4464. 10.1210/jc.2004-2140.View ArticlePubMed
- Kakinuma H, Bergert ER, Spitzweg C, Cheville JC, Lieber MM, Morris JC: Probasin promoter (ARR(2)PB)-driven, prostate-specific expression of the human sodium iodide symporter (h-NIS) for targeted radioiodine therapy of prostate cancer. Cancer Res. 2003, 63: 7840-7844.PubMed
- Scholz IV, Cengic N, Baker CH, Harrington KJ, Maletz K, Bergert ER, Vile R, Goke B, Morris JC, Spitzweg C: Radioiodine therapy of colon cancer following tissue-specific sodium iodide symporter gene transfer. Gene Ther. 2005, 12: 272-280. 10.1038/sj.gt.3302410.View ArticlePubMed
- Dwyer RM, Bergert ER, O'Connor MK, Gendler SJ, Morris JC: In vivo radioiodide imaging and treatment of breast cancer xenografts after MUC1-driven expression of the sodium iodide symporter. Clin Cancer Res. 2005, 11: 1483-1489. 10.1158/1078-0432.CCR-04-1636.View ArticlePubMed
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