Figure 2

Detection of the effects of si-CD147 on gelatinase production by gelatin zymography (A, B) and western blot assay (C, D, E). The production and expression of gelatinase were detected by gelatin zymography (A, B) and western blot assay (C, D, E), after SMMC-7721 cells were treated with si-CD147 or negative control siRNA for 48 h. MMP-2 production was decreased significantly in SMMC-7721 cells transfected with si-CD147 (A). The graph (B) compares scanning signal intensity of MMP-2 by ImageJ software. MMP-2 and MMP-9 expression greatly decreased in SMMC-7721 cells transfected with si-CD147 (C). The graph (D, E) compares scanning signal intensity of MMP-9 and MMP-2 expression by ImageJ software.