Effects of si-CD147 on FAK and vinculin expression in SMMC-7721 cells. Total cellular protein from SMMC-7721 cells tranfected with siRNA was analyzed by 10% SDS-PAGE and transferred to PVDF membrane prior to immunoblotting. The PVDF membranes were washed and the bands were scanned by Odyssey Infrared Imaging System. The results showed that si-CD147 inhibited FAK and vinculiln expression in SMMC-7721 cells (A). The graph (B) compares scanning signal intensity of FAK and vinculin expression by ImageJ software.