Figure 3
Effect of the C-terminal truncation on RON or RON160-mediated activation of downstream signaling proteins: Cells (2 × 106 cells/sample) were stimulated with RON agonistic mAb Zt/g4 (2 nM) in serum-free conditions for various times as indicated. Proteins (50 μg per lane) from cell lysates were subjected to Western blot analysis using antibodies specific to regular or phospho-p44/42, Akt, and p38 MAP kinase, respectively. Data shown here are from one of three experiments with similar results.