Figure 3
From: Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma
Immunohistochemical staining results of VEGF, NF-κB, MMP-2, MMP-9, and PCNA. VEGF, NF-κB, PCNA, MMP-2, MMP-9 protein expression including the number and staining intensity of positive cells in the treatment group were significantly lower than those in the control groups. A. VEGF expression in the control group. Tumor cells showed brown cytoplasmic staining. IHC, 400×. B. VEGF expression in the treatment group. IHC, 400×. C. NF-κB expression in the control group. Tumor cells showed brown cytoplasmic staining. The immune reaction in the nuclear was rare in this study. IHC, 400×. D. NF-κB expression in the treatment group. IHC, 400×. E. MMP-2 expression in the control group. Tumor cells showed brown cytoplasmic staining. IHC, 200×. F. MMP-2 expression in the treatment group. IHC, 200×. G. MMP-9 expression in the control group. Tumor cells showed brown cytoplasmic staining. IHC, 200×. H. MMP-9 expression in the treatment group. IHC, 200×. I. PCNA expression in the control group. Tumor cells showed brown nuclear staining. IHC, 200×. J: PCNA expression in the treatment group. IHC, 200×.