Effects of anastellin and endostatin on VEGF-dependent endothelial cell migration. Endothelial cells were seeded on collagen-coated transwell tissue culture inserts in the presence of either anastellin or endostatin, which was added to both upper and lower chambers. Control wells (C or 0) received no peptide. Positive control wells (0) were set at 1 and represent VEGF migration. After 1 hour, the 10 ng/ml VEGF was added to the lower chamber as chemoattractant. After 4 hours, the plates were fixed, stained and cells in three 10× fields counted in each of 3 membranes. The bars represent standard error of the mean from 3 separate experiments (n = 27).