Figure 1

Expression of IP-10 in vitro after transfection into COS cells. IP-10 expression in vitro and inhibition proliferation of endothelial cells by IP-10 combined with gemcitabine. A. Western blotting analysis of secreted IP-10 by COS cells. Conditioned media was obtained from COS cells transfected with empty plasmid or pBLAST-IP-10, or from non-transfected cells. The protein mass molecular markers are indicated on the right. B. Inhibition of endothelial cell proliferation by IP-10 combined with gemcitabine. Exponentially growing HUVEC, SVEC, NIH/3T3 were exposed to the conditioned medium from COS cells transfected with pBLAST-IP-10 for 72 h, or exposed to normal saline and gemcitabine. The rate of cell proliferation inhibition was calculated. Combination treatment significantly inhibited the proliferation of endothelial cells compared with gemcitabine alone, or IP-10 alone or other controls. *, P < 0.05; and **, P < 0.01.