Figure 6

Inhibition of angiogenesis assay by alginate beads in vivo. Beads containing 1 × 10⁵ Meth A cells were implanted subcutaneously into BALB/c mice on day 7 after the treatment with IP-10 plasmid or gemcitabine. Twelve days later, beads were surgically removed, and FITC-dextran was quantified. FITC-dextran uptake (E) and representative photograph of alginate beads of normal saline (A), gemcitabine (B), IP-10 plasmid (C) and IP-10 plamsid combined with gemcitabine (D). The images of alginate implants and FITC-dextran uptake showed the reduction of vascularization in combination treatment and IP-10 plasmid treated alone group compared with normal saline controls. *, P < 0.01 versus normal saline and gemcitabine group; and ^#, P < 0.01 versus IP-10 group.