Figure 3

Cilengitide inhibits proliferation and induces apoptosis in glioma cell lines G28 and G44. Proliferation assay. Cell lines G28 (A) and G44 (B) were treated with control medium or with medium containing cilengitide at the concentrations indicated and then trypsinized and counted after 24, 48 and 72 hours incubation at 37°C. Apoptosis. Floating and attached G28 (C) and G44 (D) cells were collected 24 hours after treatment with cilengitide at the indicated concentrations. Apoptosis was assessed by flow cytometry after annexin V and propidium iodide staining.