Figure 4

Cilengitide induces anoikis in G28 glioma cells. Cilengitide was added to G28 cells at the time of seeding on polyHEMA. Floating and attached cells were collected 24 hours after treatment with cilengitide at the indicated concentrations. Apoptosis was assessed by flow cytometry after annexin V and propidium iodide staining. There was no difference in rates of apoptotic cells between groups. This experiment indicates that the effect of cilengitide is comparable to other conditions inducing anoikis.