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Table 1 The primers used for real time polymerase chain reaction

From: Transcription factor Sp1 induces ADAM17 and contributes to tumor cell invasiveness under hypoxia

Gene

GenBank accession number

Sequence

HIF-1α

NM024359

5'-CGTTCCTTCGATCAGTTGTC -3'

  

5'-TCAGTGGTGGCAGTGGTAGT -3'

ADAM17

NM003183

5'-ACTCTGAGGACAGTTAACCAAACC-3'

  

5'-AGTAAAAGGAGCCAATACCACAAG-3'

Sp1

NM138473

5'-AAACATATCAAAGACCCACCAGAAT-3'

  

5'-ATATTGGTGGTAATAAGGGCTGAA-3'

TBP

NM003194

5'-TGCACAGGAGCCAAGAGTGAA-3'

  

5'-CACATCACAGCTCCCCACCA-3'

  1. ADAM17, a disintegrin and a metalloproteinase-17; HIF-1α, hypoxia inducible factor-1 alpha; Sp1, specificity transcription protein -1; TBP, TATA-binding protein.
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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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