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Table 1 The primers used for real time polymerase chain reaction

From: Transcription factor Sp1 induces ADAM17 and contributes to tumor cell invasiveness under hypoxia

Gene GenBank accession number Sequence
HIF-1α NM024359 5'-CGTTCCTTCGATCAGTTGTC -3'
   5'-TCAGTGGTGGCAGTGGTAGT -3'
ADAM17 NM003183 5'-ACTCTGAGGACAGTTAACCAAACC-3'
   5'-AGTAAAAGGAGCCAATACCACAAG-3'
Sp1 NM138473 5'-AAACATATCAAAGACCCACCAGAAT-3'
   5'-ATATTGGTGGTAATAAGGGCTGAA-3'
TBP NM003194 5'-TGCACAGGAGCCAAGAGTGAA-3'
   5'-CACATCACAGCTCCCCACCA-3'
  1. ADAM17, a disintegrin and a metalloproteinase-17; HIF-1α, hypoxia inducible factor-1 alpha; Sp1, specificity transcription protein -1; TBP, TATA-binding protein.