Tumor growth and survival rate in the subcutaneous tumor model. A. Subcutaneous tumor volume on the back at day 14 and 17 post tumor challenge. *: p < 0.01 (n = 4). Tumor volume was calculated by integration of consecutive cross-sections obtained by CT scans. B. Time course of subcutaneous tumor volume of each mouse from tumor challenge until death. Tumor growth was apparently inhibited by Tpit/E vaccination. C. Series of CT scan images showing growth of the subcutaneous tumor. (a-d): a control mouse; day 10 (a), 14 (b), 17 (c) and 21 (d) after tumor challenge, (e-h): a Tpit/E vaccinated mouse; day 10 (e), 21 (f), 24 (g) and 28 (h) after tumor challenge. Tumors were indicated by arrowheads. Arrows in panel g. and h. point to necrotic region in the tumor. D. Survival rate after tumor challenge; p < 0.05: Tpit/E vaccine vs. control.