Figure 3

Stability of hDM-αH-C6.5 MH3B1 at 37°C in the presence of serum. hDM-αH-C6.5 MH3B1 was either stored in PBS at 4°C or incubated for various times at 37°C in the presence of serum. After incubation at 37°C, fusion protein was stored at 4°C until the experiment was completed (~23 hours). hDM-αH-C6.5 MH3B1 was then added to MCF-7HER2 cells and its enzymatic stability was evaluated by its ability to convert F-dAdo to F-Ade resulting in inhibition of cellular proliferation. Data are shown as percent activity remaining of 0.001 μM of hDM-αH-C6.5 MH3B1 incubated in serum at 37°C for various times relative to the activity of 0.001 μM of hDM-αH-C6.5 MH3B1 in PBS at 4°C. The error bars represent standard deviation within each set of values.