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Table 1 Kinetic constants of fusion protein for F-dAdo as substrate.

From: Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPT

 

K M

(μM)

K cat

(s-1)

k cat /K M

(M-1s-1)

hPNP-αH-C6 MH3B1

nd

nd

Nd

hDM-αH-C6 MH3B1

264 ± 22

0.155 ± 0.017

568.2

  1. nd: no activity detected