Figure 3

Apoptosis analysis of BI-TK/GCV treated rat bladder cancer. The TUNEL assay was carried out as described in Methods. Cells with positive staining were randomly counted in 10 high-power fields and the apoptosis index was calculated (mean SD). (A) Normal saline group (6.88 ± 1.40), (B) Bifutobacterium infantis with empty plasmid group (16.01 ± 3.48), and (C) Bifutobacterium infantis-PGEX-TK group (41.72 ± 4.27). There is statistically significant difference between each groups (p < 0.05). Representative samples are shown. Magnification, 100×.