Knock-down of AEG-1 by specific siRNAs. Fourty-eight hours after transfection, cells were harvested. (A), AEG-1 mRNA levels were quantified by real-time PCR analysis. Data were normalized by using GAPDH as an internal standard. *P < 0.05 vs. parental cells. (B, C) AEG-1 protein level was analyzed by western blot. β-actin expression was monitored as the internal standard. *P < 0.05 vs. parental cells. These experiments were performed in triplicate.