AEG-1 knockdown inhibits proliferation and promotes apoptosis in neuroblastoma cells. (A, B) Cell viability was evaluated by MTT assay. The results of cell proliferation assay showed a significant decrease in the number of cells by 42.9% in M17 and 49.5% in SK-N-SH in 72 h. (C, D) Compared with that seen in the parental cells, the number of colonies was significantly reduced in the AEG-1 siRNA1 transfected group (*P < 0.05 vs. parental cells). Each experiment was performed three times in triplicate. (E) The apoptosis rate of AEG-1 siRNA1 transfected cells significantly increased by 9.6% ± 1.7% in M17 and 9.0% ± 1.4% in SK-N-SH cells (*P < 0.05 vs. parental cells), respectively. (F) Representative results are shown. These experiments were performed in triplicate.