Figure 5

Based on wound model described above, the tumor sample's immunohistochemistry analysis showed that, the TGF-β positive cells both in the wound group and the control group day 7 presented weak expression. On day 11, the wound group presented significantly strong expression of positive cells higher than the control group. The positive cells of MMP-2 and MMP-9 show the same tendency as the results in the zymography, but when the TGF-β up-regulated expression, the activity of the state of MMP-2 and MMP-9 were restored from inhibiting to the highest expression. COL IV is an important extracellular matrix, and the percentage of positive cells in the wound group found on day 7 had a lower expression compared with the control group. However, in day 11, reflected in the control group with similar results, which show that both MMPs and extracellular matrix plasticity and inflammation will continue to dampen demand in the early phase, and reach to the latter phase. This is because the cytokines such as TGF-β, play new roles on tumor cells to escape the shackles of inflammatory factors, access to the growth, and progression. A.) The positive cells are stained in brown. B.) The positive percent of cells, p < 0.01 marked by *.