Figure 5

Apoptotic changes in EC109 and EC109/R cells treated with different drugs. EC109 and EC109/R cells treated with different drugs (10 μM cisplatin, 1000 μM 5-fluorouracil, 0.6 μM doxorubicin, 0.025 μM paclitaxel or 10 μM etoposide) for 48 hours were harvested by trypsinization and subjected to annexin V/propidium iodide apoptosis detection assay using a FACS flow cytometer. The percentage of apoptotic cells was counted (Figure 3A, areas 2 and 3). Similar results were obtained in three independent experiments. Errors bar represent the standard error of the mean (p < 0.05).