Expression of PDCD4 in HCC cells. A: Immunocytochemical staining. The positive staining(×200) was brownish and localized in cytoplasm. D: Western blot assay. Representative figures are shown from one of three individual experiments. B, C or E shows statistical analysis for immunocytochemical staining, real – time PCR or western blot assay, respectively. In A, a, b or c represents cells of MHCC-97H, MHCC-97L or Hep3B, respectively; d shows cell staining without the primary antibody. In B, C and E, Group1, Group 2 or Group3 represents cells of MHCC-97H, MHCC-97L and Hep3B, respectively. Bars represent the means ± SD. The difference between Group1 and Group2 (P < 0.05) or Group1 and Group3 (P < 0.01 in B; P < 0.05 in E) was significant.