Effects of HGF and LY 294002 on Rac-1 activation. Serum-starved cells was pretreated with or without LY (10 μM) for 30 min and then treated with or without HGF. After incubation for 15 min, the cells were collected, washed, and then sonicated. Cell lysates were immunoprecipitated with PAK-1 PBD and Rac-1 activation was measured by Western blotting with a Rac-1 antibody. Representative data from three independent experiments were shown.