Serum PEDF and viral distribution in mice after Ad-PEDF treatment. A. Serum collected from mice bearing B16-F10 melanoma on day 22 after tumor inoculation was processed and subjected to an ELISA analysis to measure PEDF concentration. Compared to Ad-null or NS treated mice, serum PEDF concentration significantly increased in mice treated with Ad-PEDF (ANOVA, *, p < 0.05). B. The distribution of i.v. injected virus. The luciferase content represents the amount of virus. n = 2.