Alginate-encapsulated tumor cell assay for the inhibition of angiogenesis. Mice bearing alginate beads containing CT26 tumor cells were treated with NS, Ad-Null, or Ad-PEDF twice on day 1 and 8, respectively. On day 11, all mice received an injection of FITC-dextran and were sacrificed 20 min later. A. Photographs show surface of alginate beads from different groups. B. FITC-dextran uptake in the tumor tissue was significantly decreased in mice treated with Ad-PEDF compared to mice treated with NS (a) or Ad-Null (b) group (p < 0.05). n = 2; 4 beads/mouse.