Paclitaxel alters the expression and specific activity of deoxycytidine kinase and cytidine deaminase in non-small cell lung cancer cell lines

Table 4 The effects of paclitaxel on deoxycytdine kinase and cytidine deaminase activity and gene expression in solid tumor cell lines

Cell line	H460	H520	H838
Deoxycytidine kinase (dCK)
Control	0.46 ± 0.12	1.23 ± 0.12	2.44 ± 1.56
Paclitaxel	0.69 ± 0.14	1.67 ± 0.25	2.60 ± 0.46
Fold change	1.5	1.4^a	1.1
Cytidine deaminase (CDA)
Control	11.8 ± 3.4	18.2 ± 10.5	4.1 ± 2.1
Paclitaxel	27.0 ± 16.1	31.9 ± 11.1	10.4 ± 6.8
Fold change	2.3	1.8^a	2.5

Mean (± standard deviation) of the activity of deoxycytidine kinase (nmol per hour per 10⁶ cells) or cytidine deaminase (pmol per minute per 10⁶ cells) after exposure to vehicle-control or paclitaxel for 24 hours. The mean represents three independent experiments with each experiment conducted in at least triplicate. The fold change represents the mean activity after exposure to paclitaxel divided by the mean activity after exposure to vehicle-control.
^a P < 0.05 or P < 0.001, paclitaxel vs control-treated cells

ISSN: 1756-9966