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Table 1 Primers used for SSTRs, opioid receptors and β-actin amplification by PCR

From: Somatostatin and opioid receptors do not regulate proliferation or apoptosis of the human multiple myeloma U266 cells

Gene name

Primers

Cycles

Denaturation step

Elongation step

Anneling step

β-actin

F – 5'ATGGATGATGATATCGCCGCG3'

R-5'TCCAGACGCAGGATGGCATGG3'

35

1 min at 95°C

1 min at 72°C

1 min at 60°C

SSTR1

F-5'AGCCGGTTGACTATTACGCC3'

R-5'GCTCTCACTTCTACCATTGTC3'

45

1 min at 95°C

2 min at 72°C

1 min at 60°C

SSTR2

F-5'GGTGAAGTCCTCTGGAATCC3'

R-5'CCATTGCCAGTAGACAGAGC3'

45

30 sec at 95°C

2 min at 72°C

1 min at 63°C

SSTR3

F-5'TCATCTGCCTCTGCTACCTG3'

R-5'GAGCCCAAAGAAGGCAGGCT3'

45

30 sec at 95°C

2 min at 72°C

1 min at 65°C

SSTR4

F-5'CACCAGCGTCTTCTTCTCA3'

R-5'ATGGGGAGAGTGACCAACAG3'

35

1 min at 95°C

1 min at 72°C

1 min at 55°C

SSTR5

F-5'TCATCTGCCTGTGCTACCTG3'

R-5'GGAGAGGATGACCACGAAGA3'

35

1 min at 95°C

1 min at 72°C

1 min at 55°C

MOP-R

F-5'CAATGCAGAAGTGCCAAGAA3'

R-5'CAAGATGAAGACTGCCACCA3'

45

30 sec at 95°C

1 min at 72°C

1 min at 56°C

KOP-R

F-5'AAGGAGCACTCAATGAC3'

R-5'CAGCATCTTCACCTTGACCA3'

35

1 min at 94°C

1 min at 72°C

1 min at 55°C

DOP-R

F-5'GGACGCTGGTGGACATC3'

R-5'GGATCCCGTCTCCGAAACA3'

40

30 sec at 96°C

1 min at 72°C

30 sec at 58°C

  1. Primers (F, forward and R, reverse) used for amplification of SSTRs, opioid receptors and β-actin genes and PCR conditions are indicated.
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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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