Figure 4

Predominant Expressions of Peroxiredoxin I and Thioredoxin1 mRNA in Breast Cancer Tissue. Data in Figure 4A and Figure 4C show the transcript levels of Prx I and Trx1, respectively. The BCRT II array (qRT-PCR) was used to determine the transcript levels of Prx I-VI, Trx1, and Trx2. Data were analyzed using the comparative C_T method with the values normalized to β-actin level and expressed relative to controls. In parallel with each cDNA sample, standard curves were generated to correlate C_T values using serial dilutions of the target gene. The y-axis represents the value of pg of DNA × 10⁴. The induction fold data shown in Figure 4B and Figure 4D were obtained from the expression profiles in Figure 4A and Figure 4C, respectively. The BCRT II array consisted of five samples of normal breast tissue and 43 samples of breast cancer tissues from different individuals. Clinicopathological information for each patient was provided by the supplier. Values are reported as mean ± standard error. The t test was performed for levels of induction fold for Prx I versus other Prx isoforms (Figure 4B), and for Trx1 versus Trx2 (Figure 4D). The P values are represented by asterisks (** = P < .01, *** = P < .001). Abbreviations: BCRT II, Human Breast Cancer qRT-PCR Array II; mRNA, messenger RNA; Prx, peroxiredoxin; qRT-PCR, quantitative real-time polymerase chain reaction; Trx, thioredoxin.