Figure 5

Peroxiredoxin I and Thioredoxin1 mRNA Levels Associated with Grade of Breast Cancer. Data from the breast cancer groups using the Cancer Survey qPCR array (n = 9) and Breast Cancer qRT-PCR array I-V (n = 176) are displayed as a scatter dot plot with mean and standard error (Figure 5A). Data for induction fold for each cancer grade are represented as box-and-whisker plots with minimum and maximum. The t test was performed to compare induction fold between grade I and grade IV (Prx I, Figure 5B; Trx1, Figure 5C). The P values are represented by asterisks (** = P < .01). In addition, the Bonferroni test for multiple comparison was also performed. In this test, the P value was considered statistically significant if P < .1. The number of samples per grade and subdivided grade was distributed as follows: grade I, 37; grade II, 76 (IIA, 44; IIB, 32); grade III, 60 (IIIA, 32; IIIB, 9; IIIC, 19); and grade IV, 12. All samples in grade IV (n = 12) represent metastatic cancer. To investigate the association between induction fold and cancer grade, one-way ANOVA test for linear trend was performed between mean induction fold and subdivided cancer grades (Figure 5D). For Prx I, slope = 0.6217, P = .02; for Trx1, slope = 0.4497, P = .02. For both cases, linear trends were considered statistically significant if P < .05. Clinicopathological information for each patient was provided by the supplier. Abbreviations: ANOVA, analysis of variance; Prx I, peroxiredoxin I; qRT-PCR, quantitative real-time polymerase chain reaction; Trx1, thioredoxin 1.